嗜热栖热菌HB8中CTP合成酶的表达、纯化及初步X射线表征
Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8.
作者信息
Goto Masaru, Omi Rie, Hoseki Jun, Nakagawa Noriko, Miyahara Ikuko, Hirotsu Ken
机构信息
Department of Chemistry, Graduate School of Science, Osaka City University, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):551-3. doi: 10.1107/s0907444903000106. Epub 2003 Feb 21.
A recombinant form of the CTP synthetase from Thermus thermophilus HB8 (tCTPs) was grown as colourless crystals by the hanging-drop vapour-diffusion technique using ammonium sulfate or sodium citrate as a precipitating agent. The crystals belong to space group I222, with unit-cell parameters a = 88.2, b = 118.9, c = 142.7 A, alpha = beta = gamma = 90 degrees, and are most likely to contain a monomer in the asymmetric unit with a V(M) value of 3.07 A(3) Da(-1). The crystals obtained from ammonium sulfate and sodium citrate solutions diffract X-rays to a resolution of 2.25 A using synchrotron X-ray sources and to a resolution of 2.35 A using Cu Kalpha X-rays from a rotating-anode generator.
嗜热栖热菌HB8（tCTPs）的重组CTP合成酶通过悬滴气相扩散技术，以硫酸铵或柠檬酸钠作为沉淀剂，生长出无色晶体。这些晶体属于空间群I222，晶胞参数a = 88.2，b = 118.9，c = 142.7 Å，α = β = γ = 90°，并且在不对称单元中很可能包含一个单体，V(M)值为3.07 ų Da⁻¹。使用同步辐射X射线源时，从硫酸铵和柠檬酸钠溶液中获得的晶体将X射线衍射到2.25 Å的分辨率，使用旋转阳极发生器产生的Cu Kα X射线时，衍射分辨率为2.35 Å。
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