Takeishi Shunsaku, Nakagawa Noriko, Maoka Nobuko, Kihara Miho, Moriguchi Miki, Masui Ryoji, Kuramitsu Seiki
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1843-5. doi: 10.1107/s0907444903017712. Epub 2003 Sep 19.
Nucleoside diphosphate (NDP) kinase contributes to the maintenance of cellular pools of all nucleoside triphosphates (NTPs) by catalyzing the transfer of the gamma-phosphoryl group from an NTP donor to an NDP acceptor. NDP kinase from the extreme thermophilic bacterium Thermus thermophilus HB8 was overexpressed in Escherichia coli and crystallized at 297 K using polyethylene glycol 8000 as the precipitant by means of the hanging-drop vapour-diffusion procedure. The crystals belong to the hexagonal system, space group P6(3)22, with unit-cell parameters a = b = 124.0, c = 104.9 A, alpha = beta = 90, gamma = 120 degrees. Assuming the asymmetric unit to contain two subunits, the calculated V(M) value was 3.7 A(3) Da(-1). The crystals diffracted X-rays generated by the synchrotron at SPring-8 to at least 1.9 A resolution and were suitable for high-resolution X-ray crystal structure determination.
核苷二磷酸（NDP）激酶通过催化γ-磷酸基团从核苷三磷酸（NTP）供体转移至NDP受体，有助于维持细胞内所有核苷三磷酸（NTP）池。嗜热栖热菌HB8的NDP激酶在大肠杆菌中过表达，并通过悬滴气相扩散法，以聚乙二醇8000作为沉淀剂，在297 K下结晶。晶体属于六方晶系，空间群为P6(3)22，晶胞参数a = b = 124.0，c = 104.9 Å，α = β = 90°，γ = 120°。假设不对称单元包含两个亚基，计算得到的V(M)值为3.7 ų Da⁻¹。这些晶体对SPring-8同步加速器产生的X射线衍射，分辨率至少为1.9 Å，适合进行高分辨率X射线晶体结构测定。
