Bauer M, Schilling N, Spanel-Borowski K
Department of Anatomy, University of Leipzig, Liebigstrasse 13, 04103 Leipzig, Germany.
Cell Tissue Res. 2003 Feb;311(2):199-205. doi: 10.1007/s00441-002-0640-x. Epub 2002 Dec 4.
The corpus luteum life cycle is accompanied by capillary growth, maturation and degeneration. Arterial blood vessels are thought to undergo hyperplasia and hypertrophy during the stage of regression, as is the case with non-capillary vessels. In this study, we used morphological studies to show that the development of non-capillary vessels occurs at other corpus luteum stages. Non-capillary vessels were present at the developmental stage of the corpus luteum, and increased markedly in number in the subsequent stages. After double-staining for ASM-1 actin and Ki-67 nuclear antigen, the proliferation of smooth muscle cells (SMCs) was only detected during stages of development and secretion. When the capillaries had disappeared at the regression stage, the arterial blood-vessel walls thickened noticeably. This was attributed to the development of fibroelastosis as shown by staining for collagenous and elastic fibres. In conclusion, the bovine corpus luteum represents a physiological model for studying arteriolization at all stages of development and secretion. At the regression stage, arterioregression sets in.
黄体生命周期伴随着毛细血管的生长、成熟和退化。动脉血管在退化阶段被认为会发生增生和肥大，非毛细血管也是如此。在本研究中，我们通过形态学研究表明，非毛细血管的发育发生在黄体的其他阶段。非毛细血管在黄体发育阶段就已存在，且在随后的阶段数量显著增加。对平滑肌肌动蛋白（ASM-1）和Ki-67核抗原进行双重染色后，仅在发育和分泌阶段检测到平滑肌细胞（SMC）的增殖。当退化阶段毛细血管消失时，动脉血管壁明显增厚。这归因于胶原纤维和弹性纤维染色显示的纤维弹性组织增生。总之，牛黄体是研究发育和分泌各阶段动脉化的生理模型。在退化阶段，动脉退化开始。
