用钴（III）螯合物对肽和蛋白质的N端氨基酸进行定量测定。
Quantitative determination of N-terminal amino acids of peptides and proteins with cobalt(III) chelates.
作者信息
Bentley K W
出版信息
Biochem J. 1976 Jan 1;153(1):137-8. doi: 10.1042/bj1530137.
Abstract
Quantitative N-terminal peptide-bond hydrolysis with the cis-beta-hydroxyaquo(triethylenetetramine) cobal (III) ion, i.e. beta-[Co(trien)(OH)(OH2)]2+, is reported. The method has been demonstrated with 20 small peptides, a hexapeptide, bradykinin, insulin A chain (oxidized), glucagon and insulin. The procedure involves no acidic hydrolysis step and thus no destruction of labile amino acids.
摘要
报道了用顺式-β-羟基水合（三亚乙基四胺）钴（III）离子，即β-[Co（trien）（OH）（OH₂）]²⁺进行N端肽键的定量水解。该方法已在20种小肽、一种六肽、缓激肽、胰岛素A链（氧化型）、胰高血糖素和胰岛素上得到验证。该程序不涉及酸性水解步骤，因此不会破坏不稳定的氨基酸。
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本文引用的文献
1
Hydrolysis of N-terminal peptide bonds and amino acid derivatives by the beta-hydroxoaquotriethylenetetraminecobalt (3) ion.β-羟基水合三亚乙基四胺钴（3）离子对N-末端肽键和氨基酸衍生物的水解作用。
J Am Chem Soc. 1967 Mar 1;89(5):1082-7. doi: 10.1021/ja00981a008.
2
Qualitative determination of N-terminal amino acids of peptides and proteins with cobalt(3) chelates.用钴（Ⅲ）螯合物对肽和蛋白质的N端氨基酸进行定性测定。
Biochem J. 1973 Nov;135(3):507-11. doi: 10.1042/bj1350507.
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