Mehra A, Gupta S C, Narang C K, Mathur N K
Indian J Biochem Biophys. 1989 Oct;26(5):348-9.
Cellulose was functionalized to incorporate triethylenetetramine group. This was in turn converted into the polymeric analogue of cobalt(III)triene complex. The polymeric complex reacts with peptides resulting in the cleavage of amino end amino acid, thus suggesting the applicability of the polymeric reagent as a solid phase reagent for N-terminal determination.
纤维素经过功能化处理以引入三亚乙基四胺基团。该基团进而被转化为钴(III)三烯配合物的聚合物类似物。该聚合物配合物与肽发生反应,导致氨基末端氨基酸的裂解,从而表明该聚合物试剂作为用于N端测定的固相试剂的适用性。