Jiang Zhong-Qing, Zhu Feng-Chuan, Qu Jun-Ying, Zheng Xiu, You Cai-Ling
Department of Obstetrics and Gynecology, The Affiliated First Hospital, Fujian Medical University, Fuzhou, Fujian, 350005, PR China.
Ai Zheng. 2003 Feb;22(2):178-84.
BACKGROUND & OBJECTIVE: Matrix metalloproteinase-9 (MMP-9) might plays an important role in regulating processes of tumor angiogenesis, invasion,and metastasis. The expression and clinical significance of MMP-9 in early invasive carcinoma of cervix was investigated.
Expression of MMP-9, microvessel density (MVD) labeled by CD(34) and proliferation index of cancer cells labeled by Ki-67 in 75 cases of early invasive carcinoma of cervix (ICC), 18 cases of cervical intraepithelial neoplasm (CIN), and 15 cases of normal cervical epithelium (NCE) were detected by immunohistochemistry SP method.
MMP-9 was mainly expressed in the cellular membrane and/or cytoplasm in tumor cells,whereas expression of Ki-67 was mainly confined to the nuclei, and that of CD(34), to the vascular epithelial cells in tumor stroma. It was shown that positive rate of expression of MMP-9,Ki-67, and MVD increased remarkably from NCE to CIN,and then to ICC accordingly(P< 0.05). In ICC, MVD was positively related to the expression of MMP-9 (r=0.287, P<0.05). The expression of MMP-9 correlated with pelvic lymph node metastasis, intravascular and stromal infiltration, FIGO staging, histological grading,and Ki-67 expression (P< 0.05 or P< 0.01); but not correlated with patients age and histological types (P >0.05). In cases with pelvic lymph node metastasis, intravascular and deeper stroma infiltration, FIGO staging II, histological grade III and over-expression of Ki-67, positive rate of expression of MMP-9 was significantly higher than that in those without the conditions mentioned above (P< 0.05).
In cervical carcinoma, MMP-9 may play an important role during tumor angiogenesis, proliferation of cancer cells, cancer invasion and metastasis. Over-expression of MMP-9 may result in great increase of tumor angiogenesis, rapid proliferation of cancer cells, cancer invasion and metastasis, but it is not the only decisive factor. Detection expression of MMP-9 may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.
基质金属蛋白酶-9（MMP-9）可能在肿瘤血管生成、侵袭及转移过程中发挥重要作用。本研究探讨MMP-9在宫颈早期浸润癌中的表达及其临床意义。
采用免疫组织化学SP法检测75例宫颈早期浸润癌（ICC）、18例宫颈上皮内瘤变（CIN）及15例正常宫颈上皮（NCE）中MMP-9的表达、CD34标记的微血管密度（MVD）及Ki-67标记的癌细胞增殖指数。
MMP-9主要表达于肿瘤细胞的细胞膜和/或细胞质，Ki-67的表达主要局限于细胞核，CD34的表达主要位于肿瘤间质中的血管内皮细胞。结果显示，从NCE到CIN再到ICC，MMP-9、Ki-67的表达阳性率及MVD均显著升高（P<0.05）。在ICC中，MVD与MMP-9的表达呈正相关（r=0.287，P<0.05）。MMP-9的表达与盆腔淋巴结转移、血管及间质浸润、国际妇产科联盟（FIGO）分期、组织学分级及Ki-67的表达相关（P<0.05或P<0.01）；但与患者年龄及组织学类型无关（P>0.05）。在有盆腔淋巴结转移、血管及深层间质浸润、FIGO分期II期、组织学分级III级及Ki-67过表达的病例中，MMP-9的表达阳性率显著高于无上述情况者（P<0.05）。
在宫颈癌中，MMP-9可能在肿瘤血管生成、癌细胞增殖、侵袭及转移过程中发挥重要作用。MMP-9的过表达可能导致肿瘤血管生成显著增加、癌细胞快速增殖、侵袭及转移，但并非唯一的决定性因素。检测MMP-9的表达可能有助于进一步了解宫颈癌的生物学行为并预测其预后。
