Ribaya-Mercado Judy D, Solon Florentino S, Dallal Gerard E, Solomons Noel W, Fermin Liza S, Mazariegos Manolo, Dolnikowski Gregory G, Russell Robert M
Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA. judy.ribaya-mercado.tufts.edu
Am J Clin Nutr. 2003 Mar;77(3):694-9. doi: 10.1093/ajcn/77.3.694.
The conventional deuterated-retinol-dilution (DRD) technique provides a quantitative estimate of total body stores of vitamin A in humans. The procedure requires equilibration of serum deuterated retinol with nondeuterated retinol after administration of an oral dose of deuterated vitamin A. Equilibration takes approximately 3 wk to complete.
Our goal was to develop a predictive mathematical formula for quantitative assessment of total body stores of vitamin A in adults by using a procedure that takes less time to perform because serum isotope equilibration is not required, so that blood drawing can be done 3 d, instead of approximately 3 wk, after isotope dosing.
Ratios of serum deuterated to nondeuterated retinol (D:H retinol) were determined in Filipino adults (n = 68) 3 and 20 d after an oral dose of 0.015 mmol [(2)H(4)]retinyl acetate and in Guatemalan adults (n = 15) 3 and 21 d after a 0.030-mmol dose. D:H retinol values 20 or 21 d after the isotope dose were used in a mathematical formula to obtain quantitative estimates of total body stores of vitamin A that were then correlated with serum D:H retinol values 3 d after the isotope dose.
The relation between these variables was nonlinear and was described by the following equation: total body stores of vitamin A (in mmol retinol) = 0.00468 x 10(37(isotope dose in mmol))/D:H retinol in serum 3 d after the isotope dose.
A 3-d DRD technique could be used for quantitative assessment of total body stores of vitamin A; this technique takes less time than does the conventional DRD technique.
传统的氘代视黄醇稀释（DRD）技术可对人体维生素A的全身储备进行定量评估。该方法需要在口服氘代维生素A后，使血清中的氘代视黄醇与未氘代的视黄醇达到平衡。平衡过程大约需要3周才能完成。
我们的目标是开发一种预测性数学公式，通过一种耗时较短的方法对成年人维生素A的全身储备进行定量评估，因为该方法不需要血清同位素平衡，所以在同位素给药后3天而不是大约3周即可进行采血。
在菲律宾成年人（n = 68）口服0.015 mmol [（2）H（4）]醋酸视黄酯后3天和20天，以及危地马拉成年人（n = 15）口服0.030 mmol剂量后3天和21天，测定血清中氘代视黄醇与未氘代视黄醇的比例（D:H视黄醇）。将同位素给药后20天或21天的D:H视黄醇值用于一个数学公式，以获得维生素A全身储备的定量估计值，然后将其与同位素给药后3天的血清D:H视黄醇值进行关联。
这些变量之间的关系是非线性的，可用以下方程描述：维生素A的全身储备（以视黄醇毫摩尔计）= 0.00468 x 10（37（同位素剂量，单位为毫摩尔））/同位素给药后3天血清中的D:H视黄醇。
3天DRD技术可用于维生素A全身储备的定量评估；该技术比传统DRD技术耗时更少。
