尿液和重组促红细胞生成素的毛细管电泳图谱差异。
Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin.
作者信息
De Frutos Mercedes, Cifuentes Alejandro, Diez-Masa Jose C
机构信息
Institute of Organic Chemistry, CSIC, Madrid, Spain.
出版信息
Electrophoresis. 2003 Feb;24(4):678-80. doi: 10.1002/elps.200390080.
Different profiles were obtained by capillary zone electrophoresis (CZE) of human erythropoietin (EPO) of recombinant and urinary origin. To unambiguously detect doping by EPO, direct methods able to determine the presence of the drug itself in a physiological fluid are required. Since the host cell line used for EPO production influences its glycosylation, the carbohydrate distribution of natural human EPO may be different from that of recombinant EPO. The different content in sialic acid groups between recombinant and endogenous EPO provide a basis for their distinction by CZE.
通过毛细管区带电泳（CZE）对重组来源和尿源的人促红细胞生成素（EPO）进行分析，得到了不同的图谱。为了明确检测EPO兴奋剂，需要能够直接测定生理体液中药物本身存在的方法。由于用于生产EPO的宿主细胞系会影响其糖基化，天然人EPO的碳水化合物分布可能与重组EPO不同。重组EPO和内源性EPO之间唾液酸基团含量的差异为通过CZE区分它们提供了依据。
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