用于检测促红细胞生成素兴奋剂的超滤尿液的成功DNA分型。

Successful DNA typing of ultrafiltered urines used to detect EPO doping.

作者信息

Castella V, Morerod M-L, Robinson N, Saugy M, Mangin P

机构信息

Laboratoire de Génétique Forensique, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland.

出版信息

Forensic Sci Int Genet. 2007 Dec;1(3-4):281-2. doi: 10.1016/j.fsigen.2007.02.010. Epub 2007 Mar 23.

DOI:10.1016/j.fsigen.2007.02.010

PMID:19083774

Abstract

Endogenous and exogenous erythropoietin (EPO) present in urine can be distinguished according to their isoelectric profiles. This methodology requires urine samples to be concentrated about 200 to 1000 times with manipulations that should remove most of the cells occurring in the original sample. In this study, we tried to obtain DNA profiles from 10 ultrafiltered urines (retentates) in order to evaluate whether a formal genetic identification was technically feasible. No nuclear DNA profiles could be established from retentates, despite 34 PCR-cycles amplifications. Contrastingly, mitochondrial DNA (mtDNA) profiles were obtained for 9 out of the 10 retentates. Apart from some particularities, retentate mtDNA profiles were all distinct and matched mtDNA profiles of corresponding reference samples.

摘要

尿液中存在的内源性和外源性促红细胞生成素（EPO）可根据其等电谱进行区分。该方法要求对尿液样本进行约200至1000倍的浓缩，操作过程应去除原始样本中出现的大多数细胞。在本研究中，我们试图从10份超滤尿液（截留物）中获取DNA谱，以评估正式的基因鉴定在技术上是否可行。尽管进行了34个PCR循环的扩增，但仍无法从截留物中建立核DNA谱。相比之下，10份截留物中有9份获得了线粒体DNA（mtDNA）谱。除了一些特殊性外，截留物的mtDNA谱均各不相同，且与相应参考样本的mtDNA谱匹配。