Zhang Guodong, Li Yongshan, Fang Yi'ou, Han Nanyin, Xu Bingjiu
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Electrophoresis. 2003 Feb;24(4):693-9. doi: 10.1002/elps.200390083.
Octadecyl silyl silica (ODS) phase coated with immobilized polysiloxanes (OV1701, SE-54, SE-30) were synthesized, their characteristics as capillary electrochromatography (CEC) column packing materials were studied. It was found that, although the polysiloxane coatings were different in polarity, the resulting packing materials showed the highest efficiencies when the respective coating ratios (polysiloxane:ODS, w/w) were all 20-30%. As expected, packing materials coated with different polysiloxanes resulted in different selectivity on solute pairs. Separations on these stationary phases were studied with different factors such as pH values and acetonitrile contents of the mobile phases. It was found that all these kind of stationary phases could resist basic mobile phase with a pH value as high as 11.6. Tests were made to analyze polar, basic drugs with CEC using the stationary phases.
合成了涂覆有固定化聚硅氧烷（OV1701、SE - 54、SE - 30）的十八烷基硅烷硅胶（ODS）相，并研究了它们作为毛细管电色谱（CEC）柱填充材料的特性。结果发现，尽管聚硅氧烷涂层的极性不同，但当各自的涂层比例（聚硅氧烷：ODS，w/w）均为20 - 30%时，所得填充材料表现出最高的效率。正如预期的那样，涂覆不同聚硅氧烷的填充材料对溶质对具有不同的选择性。研究了这些固定相在不同因素（如流动相的pH值和乙腈含量）下的分离情况。结果发现，所有这些类型的固定相都能耐受pH值高达11.6的碱性流动相。使用这些固定相对极性、碱性药物进行了毛细管电色谱分析测试。
