Norton Dean, Shamsi Shahab A
Department of Chemistry, Center of Biotechnology and Drug Design, Georgia State University, Atlanta, GA 30303, USA.
Electrophoresis. 2004 Feb;25(4-5):586-93. doi: 10.1002/elps.200305682.
Nonionic surfactants such as Triton X-100 (TX-100) are comprised of a mixture of oligomers with a varying degree of length in the ethoxylate chain. The development of chromatographic methods for resolution of the various oligomers of TX-100 is of environmental importance, and can be useful for quality control and characterization in industrial manufacture. Capillary electrochromatography (CEC) is fast becoming a capable separation technique that combines the benefits of both high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). This report presents a novel CEC method for separation of the various TX-100 oligomers. A comparison of monomeric vs. polymeric stationary phases for separation of TX-100 was conducted. Since the oligomers of TX-100 were better resolved on a monomeric phase as compared to polymeric phase, a systematic mobile phase tuning was performed utilizing a monomeric CEC-C18-3 microm-100 A stationary phase. Various mobile phase parameters such as acetonitrile (ACN) content, Tris concentration, pH, voltage, and temperature were manipulated in order to achieve the optimum separation of oligomers in less than 30 min.
非离子表面活性剂，如 Triton X-100（TX-100），由乙氧基链长度不同的低聚物混合物组成。开发用于分离 TX-100 各种低聚物的色谱方法具有环境重要性，并且可用于工业生产中的质量控制和特性表征。毛细管电色谱（CEC）正迅速成为一种强大的分离技术，它结合了高效液相色谱（HPLC）和毛细管电泳（CE）的优点。本报告介绍了一种用于分离各种 TX-100 低聚物的新型 CEC 方法。对用于分离 TX-100 的单体与聚合物固定相进行了比较。由于与聚合物相比，TX-100 的低聚物在单体相上的分离效果更好，因此利用单体 CEC-C18-3 微米-100 A 固定相进行了系统的流动相调节。为了在不到 30 分钟的时间内实现低聚物的最佳分离，对各种流动相参数，如乙腈（ACN）含量、Tris 浓度、pH、电压和温度进行了调整。
