[杭白菊对牛主动脉平滑肌细胞凋亡的影响]
[Effect of Chrysanthemum morifolium Ramat on apoptosis of bovine aortic smooth muscle cells].
作者信息
Fang Xue-Ling, Wang Xi-Tian, Huang Shu-Ru, Li Xia
机构信息
The First Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310003, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002 Aug;31(5):347-350. doi: 10.3785/j.issn.1008-9292.2002.05.009.
OBJECTIVE
To observe the effect and the mechanism of Chrysanthemum morifolium Ramat on apoptosis of bovine aortic smooth muscle cells. METHODS: Vascular smooth muscle cells were isolated from thoracic aorta of fetal calf and cultured, then incubated with different concentration of Chrysanthemum morifolium Ramat. Apoptosis was measured by flow cytometry. SOD and MDA were measured by spectrophotometer. RESULTS: We found that: (1) the number of apoptotic cells was reduced from (4.425+/-0.624)% to (2.875+/-0.640)% in Chrysanthemum morifolium Ramat group, in a concentration dependent manner; (2) the value of SOD was increased from (1.683+/-0.149)X10(4) U/L to (2.297+/-0.230)X104 U/L and the value of MDA was reduced from(166.454+/-56.805)&mgr;mol/L to (73.068+/-27.203)&mgr;mol/L in Chrysanthemum morifolium Ramat group, also in a concentration dependent manner. CONCLUSION: Chrysanthemum morifolium Ramat can inhibit apoptosis of vascular smooth muscle cells in a concentration-dependent manner.
目的
观察杭白菊对牛主动脉平滑肌细胞凋亡的影响及其机制。方法：从胎牛胸主动脉分离培养血管平滑肌细胞，然后用不同浓度的杭白菊进行孵育。采用流式细胞术检测细胞凋亡。用分光光度计检测超氧化物歧化酶（SOD）和丙二醛（MDA）。结果：我们发现：（1）杭白菊组凋亡细胞数量从（4.425±0.624）%降至（2.875±0.640）%，呈浓度依赖性；（2）杭白菊组SOD值从（1.683±0.149）×10⁴U/L升至（2.297±0.230）×10⁴U/L，MDA值从（166.454±56.805）μmol/L降至（73.068±27.203）μmol/L，也呈浓度依赖性。结论：杭白菊可呈浓度依赖性抑制血管平滑肌细胞凋亡。
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