Gil Geun-Cheol, Chang In-Seop, Kim Byung-Hong, Kim Mia, Jang Jae-Kyung, Park Hyung Soo, Kim Hyung-Joo
Water Environment Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok, Sungpook, Seoul 136-791, South Korea.
Biosens Bioelectron. 2003 Apr;18(4):327-34. doi: 10.1016/s0956-5663(02)00110-0.
A mediator-less microbial fuel cell was optimized in terms of various operating conditions. Current generation was dependent on several factors such as pH, resistance, electrolyte used, and dissolved oxygen concentration in the cathode compartment. The highest current was generated at pH 7. Under the operating conditions, the resistance was the rate-determining factor at over 500 omega. With resistance lower than 500 omega, proton transfer and dissolved oxygen (DO) supply limited the cathode reaction. A high strength buffer reduced the proton limitation to some extent. The DO concentration was around 6 mg l(-1) at the DO limited condition. The fact that oxygen limitation was observed at high DO concentration is believed to be due to the poor oxygen reducing activity of the electrode used, graphite. The current showed linear relationship with the fuel added at low concentration, and the electronic charge was well correlated with substrate concentration from up to 400 mg l(-1) of COD(cr). The microbial fuel cell might be used as a biochemical oxygen demand (BOD) sensor.
对无介体微生物燃料电池的各种运行条件进行了优化。电流的产生取决于几个因素，如pH值、电阻、所用电解质以及阴极室中的溶解氧浓度。在pH值为7时产生的电流最高。在运行条件下，电阻在超过500欧姆时是速率决定因素。当电阻低于500欧姆时，质子转移和溶解氧（DO）供应限制了阴极反应。高强度缓冲剂在一定程度上降低了质子限制。在溶解氧受限的条件下，溶解氧浓度约为6mg l(-1)。在高溶解氧浓度下观察到氧限制这一事实被认为是由于所用电极（石墨）的氧还原活性较差。在低浓度下，电流与添加的燃料呈线性关系，并且从高达400mg l(-1)的化学需氧量（COD(cr)）来看，电荷量与底物浓度具有良好的相关性。微生物燃料电池可作为生化需氧量（BOD）传感器使用。
