Enhancing biohydrogen production from mono-substrates and co-substrates using a novel bacterial strains.

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The staggering increase in pollution associated with a sharp tightening in global energy demand is a major concern for organic substances. Renewable biofuel production through simultaneous waste reduction is a sustainable approach to meet this energy demand. This study co-fermentation of dairy whey and SCB was performed using mixed and pure bacterial cultures of , , and by dark fermentation process for hydrogen production. The maximum H production was 202.7 ± 5.5 H/mL/L, 237.3 ± 6.0 H/mL/L, and 198 ± 9.9 H/mL/L obtained in fermentation reactions containing dairy whey, solid and liquid hydrolysis of pretreated sugarcane bagasse as mono-substrates. The H production was greater in co-substrate by 347.3 ± 18.5 H/mL/L under optimized conditions (pH 7.0, temperature 37 °C, substrate concentration 30:50 g/L) than expected in mono-substrate conditions, which confirms that co-fermentation of different substrates enhances the H potential. Fermentation medium during bio-H2 production under GC analysis has stated that using mixed cultures in dark fermentation favored acetic acid and butyric acid. Co-substrate degradation produces ethyl alcohol, benzoic acid, propionic acid, and butanol as metabolic by-products. The difference in the treated and untreated substrate and carbon enrichment in the substrates was evaluated by FT-IR analysis. The present study justifies that rather than the usage of mono-substrate for bio-H production, the co-substrate provided highly stable H production by mixed bacterial cultures. Fabricate the homemade single-chamber microbial fuel cell to generate electricity.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-023-03687-9.