Ehrentreich-Förster Eva, Scheller Frieder W, Bier Frank F
Department Molecular Bioanalytics, Fraunhofer Institute for Biomedical Engineering, Arthur-Scheunert-Allee 114-116, 14558 Bergholz-Rehbrücke, Germany.
Biosens Bioelectron. 2003 Apr;18(4):375-80. doi: 10.1016/s0956-5663(02)00145-8.
The progesterone concentration in blood samples can be utilised as a marker for the diagnosis of early pregnancy, endocrinopathy and virilism. Here, we describe a method for progesterone detection and measurement in whole blood samples by a surface sensitive biosensor used in conjunction with an integrated optical grating coupler. This device determines refractive index changes near the biosensor's surface. Hence, biological species bound to a surface layer can be measured in real-time without any label. For the measurements, we have modified the indirect competitive immunoassay principle. The concentration of the progesterone antibody was kept at 1 microg/ml. Progesterone concentration was determined in buffer solution and whole blood in a range between 0.005 and 10 ng/ml. The detection limit was determined to be 3 pM. The relative standard deviation was calculated to be 3.5%.
血液样本中的孕酮浓度可作为早期妊娠、内分泌病和男性化诊断的标志物。在此，我们描述了一种通过与集成光栅耦合器结合使用的表面敏感生物传感器检测和测量全血样本中孕酮的方法。该设备可确定生物传感器表面附近的折射率变化。因此，无需任何标记即可实时测量与表面层结合的生物物种。对于测量，我们修改了间接竞争免疫分析原理。孕酮抗体的浓度保持在1微克/毫升。在缓冲溶液和全血中测定孕酮浓度，范围在0.005至10纳克/毫升之间。检测限确定为3皮摩尔。计算相对标准偏差为3.5%。
