Nabok A V, Tsargorodskaya A, Holloway A, Starodub N F, Gojster O
Sheffield Hallam University, Materials and Engineering Research Institute, City Campus, Pond Street, Sheffield S1 1WB, UK.
Biosens Bioelectron. 2007 Jan 15;22(6):885-90. doi: 10.1016/j.bios.2006.03.010. Epub 2006 Apr 19.
A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 microg/ml down to 0.15 ng/ml. Association constants of 1.4x10(6) and 1.9x10(7)mol(-1)s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4-5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies.
一种结合免疫分析方法的全内反射椭圆偏振光谱法（TIRE）的灵敏光学方法被用于检测浓度范围从100微克/毫升到0.15纳克/毫升的T-2霉菌毒素。通过TIRE动力学测量分别评估了多克隆和单克隆T-2抗体的缔合常数，分别为1.4×10⁶和1.9×10⁷摩尔⁻¹秒⁻¹。根据TIRE数据拟合，T-2分子与抗体的结合（饱和时）导致吸附层厚度增加了4-5纳米。石英晶体微天平阻抗测量数据显示质量异常大幅增加和薄膜软化，这很可能是由于大的T-2聚集体与抗体结合所致。
