Horváth R, Fricsovszky G, Papp E
Department of Biological Physics, Eötvös University, Pázmány Péter sétány 1A, 1113 Budapest, Hungary.
Biosens Bioelectron. 2003 Apr;18(4):415-28. doi: 10.1016/s0956-5663(02)00154-9.
An instrument for optical waveguide lightmode spectroscopy (OWLS) was designed and developed for measurements at different and controlled temperatures in a range of 15 degrees C around room temperature. The instrument allows to scan the waveguide modes at different wavelengths on the same optical chip using different lasers. This instrument was used to monitor DMPC lipid bilayer main phase transition around the critical temperature. The main problem in these experiments is that the OWLS measurements do not give enough information about an optically anisotropic system like a lipid bilayer. Experimental OWLS data at two different wavelengths can however approximately solve the problem. The temperature dependence of the thickness and the refractive indices (ordinary and extraordinary) for the lipid bilayer around the phase transition is presented. (A theoretical derivation of the extraordinary refractive index is given in.)
设计并开发了一种用于光波导光模式光谱（OWLS）的仪器，用于在室温附近15摄氏度范围内的不同且可控温度下进行测量。该仪器允许使用不同的激光器在同一光学芯片上扫描不同波长的波导模式。该仪器用于监测二肉豆蔻酰磷脂酰胆碱（DMPC）脂质双层在临界温度附近的主要相变。这些实验中的主要问题是，OWLS测量无法提供关于脂质双层这样的光学各向异性系统的足够信息。然而，在两个不同波长下的实验OWLS数据可以大致解决这个问题。给出了相变附近脂质双层厚度和折射率（寻常折射率和非寻常折射率）的温度依赖性。（文中给出了非寻常折射率的理论推导。）
