Scarpace P J, Neuman W F
Calcif Tissue Res. 1976 Apr 20(2):137-49.
The role of periosteal and endosteal cellular envelopes in the control of the K+ and Na+ content of embryonic chick calvaria was investigated. Data have been presented which suggest that the K+ in extracellular fluid of calvaria may be under a direct membrane control similar to the pump-leak systems hypothesized for most cells. Based on DNA and K+ analysis of cell water, volume and DNA content, the K+ in the bone extracellular fluid was calculated to account for 75% of the total K+ content of intact calvaria. The bulk of K+ is therefore extracellular and its accumulation there was found to be sensitive to the presence of ouabain, iodoacetate, CCCP, and to temperature. Losses of 70% of calvaria K+ were found one hour after addition of 250 muM ouabain. If the exposure to ouabain was brief, the K+ loss was partially reversed. The rate of 42K+ uptake was found to be depressed by ouabain, decreased pH, and La2+. The total Na+ content of these calvaria was found not to vary when incubated with ouabain, iodoacetate, or CCCP.
研究了骨膜和骨内膜细胞包膜在控制胚胎鸡颅骨钾离子（K⁺）和钠离子（Na⁺）含量中的作用。已呈现的数据表明，颅骨细胞外液中的K⁺可能受直接的膜控制，类似于大多数细胞所假设的泵 - 漏系统。基于对细胞水、体积和DNA含量的DNA和K⁺分析，计算出骨细胞外液中的K⁺占完整颅骨总K⁺含量的75%。因此，大部分K⁺位于细胞外，并且发现其在那里的积累对哇巴因、碘乙酸盐、碳酰氰间氯苯腙（CCCP）的存在以及温度敏感。添加250μM哇巴因一小时后，发现颅骨K⁺损失了70%。如果对哇巴因的暴露时间短暂，K⁺损失会部分逆转。发现哇巴因、降低的pH值和La²⁺会抑制⁴²K⁺的摄取速率。当与哇巴因、碘乙酸盐或CCCP一起孵育时，发现这些颅骨的总Na⁺含量没有变化。
