Brommage R, Neuman W F
Calcif Tissue Int. 1979 Aug 24;28(1):57-63. doi: 10.1007/BF02441218.
Four-day-old chick calvaria were used to determine the passive concentrations of magnesium, sodium, and potassium in metabolically poisoned bone. When incubated in buffers containing the blood levels of sodium and magnesium, these calvaria contained sodium and magnesium at the identical concentrations found in freshly dissected calvaria. Calvarial sodium and magnesium levels could be varied by altering the buffer concentrations of these cations. The potassium content of metabolically poisoned calvaria incubated in buffers containing 4 mM potassium was less than 20% of the content of freshly dissected calvaria. When the buffer concentrations of sodium and potassium were systematically varied, ouabain-poisoned calvaria concentrated these cations in the bone extracellular fluid by a factor of approximately two above buffer cation levels. Presumably, the hydroxyapatite crystal zeta potential is responsible for this concentrative phenomenon. These results are discussed in terms of the control of the ionic content of the bone extracellular fluid by the postulated "bone membrane."
用4日龄雏鸡颅骨来测定代谢中毒骨骼中镁、钠和钾的被动浓度。当在含有血液水平钠和镁的缓冲液中孵育时，这些颅骨所含钠和镁的浓度与刚解剖的颅骨中发现的浓度相同。通过改变这些阳离子的缓冲液浓度，可以改变颅骨的钠和镁水平。在含有4 mM钾的缓冲液中孵育的代谢中毒颅骨的钾含量不到刚解剖颅骨含量的20%。当系统地改变钠和钾的缓冲液浓度时，哇巴因中毒的颅骨将这些阳离子在骨细胞外液中的浓度浓缩至比缓冲液阳离子水平高约两倍。据推测，羟基磷灰石晶体的ζ电位是这种浓缩现象的原因。根据假定的“骨膜”对骨细胞外液离子含量的控制来讨论这些结果。
