硫酸葡聚糖和肝素多阴离子预处理靶细胞可消除类抑素样效应。
Chalone-like effect abrogated by dextran sulphate and heparin polyanion pretreatment of target cells.
作者信息
Ebbesen P, Olsson L
出版信息
Br J Cancer. 1975 Jun;31(6):649-52. doi: 10.1038/bjc.1975.111.
Abstract
Chalone prepared from primary BALB/c mouse embryo fibroblasts caused a 33% reduction in incorporation of tritiated thymidine in the cultures of the second in vitro passage of BALB/c embryo fibroblasts, whereas chalone prepared from thymus, skin and spleen was without effect. Pretreatment of BALB/c secondary fibroblasts with the polyanions dextran sulphate and heparin abrogated the chalone effect. The polycations DEAE-dextran and polybrene were without effect. The effect of incubation with the dextran sulphate polyanion was reversed when followed by incubation with DEAE-dextran polycation.
摘要
从原代BALB/c小鼠胚胎成纤维细胞制备的抑素，使BALB/c胚胎成纤维细胞体外传代培养第二代的氚标记胸腺嘧啶核苷掺入量减少了33%，而从胸腺、皮肤和脾脏制备的抑素则无此作用。用聚阴离子硫酸葡聚糖和肝素对BALB/c二代成纤维细胞进行预处理可消除抑素的作用。聚阳离子二乙氨基乙基葡聚糖和聚凝胺则无此作用。在用硫酸葡聚糖聚阴离子孵育后再用二乙氨基乙基葡聚糖聚阳离子孵育，其作用可逆转。
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Chalone-like effect abrogated by dextran sulphate and heparin polyanion pretreatment of target cells.硫酸葡聚糖和肝素多阴离子预处理靶细胞可消除类抑素样效应。
Br J Cancer. 1975 Jun;31(6):649-52. doi: 10.1038/bjc.1975.111.
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Inhibitory effect of polycations on the transplantability of mouse leukaemia reversed by heparin.多阳离子对小鼠白血病移植性的抑制作用被肝素逆转。
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Delayed inhibition of epidermal DNA synthesis after injection of an aqueous skin extract (chalone).注射皮肤水提取物（抑素）后表皮DNA合成的延迟抑制
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Influence of DEAE-dextran, polybrene, dextran and dextran sulphate on spontaneous leukaemia development in AKR mice and virus induced leukaemia in BALB-c mice.二乙氨基乙基葡聚糖、聚凝胺、葡聚糖和硫酸葡聚糖对AKR小鼠自发性白血病发生及BALB - c小鼠病毒诱导白血病的影响。
Br J Cancer. 1974 Jul;30(1):68-72. doi: 10.1038/bjc.1974.114.
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