Wu Maoxin, Putti Thomas C, Bhuiya Tawfiqul A
Department of Pathology, Mount Sinai Hospital, Mount Sinai School of Medicine, New York, New York, USA.
Appl Immunohistochem Mol Morphol. 2002 Dec;10(4):351-6. doi: 10.1097/00129039-200212000-00011.
Verrucous carcinoma (VC) is a locally invasive, nonmetastasizing variant of squamous cell carcinoma (SCC) with distinct clinical and histologic features. Molecular alterations detectable by immunohistochemical analyses in VC have not been extensively studied. This study investigates the expression of p53, epidermal growth factor receptor (EGFR), transforming growth factor-alpha (TGF-alpha), and cyclin D1 in VC, verrucous hyperplasia (VH), and classic SCC of the head and neck. Twenty-six cases of VC, 12 cases of SCC of various differentiations, and 4 cases of VH were studied. Formalin-fixed, paraffin-embedded archival material was used for immunohistochemistry (avidin-biotin immunoperoxidase technique) to study the expression of oncogenes and their tumor markers. Identification of p53 protein was found in 100% of VH, 88% of VC, and 100% of SCC. EGFR expression was noted in 25% of VH, 54% of VC, 40% of well-differentiated SCC (WDSCC), and 100% of moderately and poorly differentiated SCC (MDSCC/PDSCC). TGF-alpha was detected in 25% of VH, 88% of VC, 80% WDSCC, and 100% of MDSCC/PDSCC. Cyclin-D1 expression was seen in 75% of VH, 35% of VC, 100% of WDSCC, 67% of MDSCC, and 50% of PDSCC. Correlation between the level of expression of all markers and the grade of this group of squamous lesions revealed statistically significant correlation coefficients for p53 and EGFR but not for TGF-alpha and cyclin D1.
疣状癌（VC）是鳞状细胞癌（SCC）的一种局部浸润性、无转移的变体，具有独特的临床和组织学特征。通过免疫组织化学分析在VC中可检测到的分子改变尚未得到广泛研究。本研究调查了p53、表皮生长因子受体（EGFR）、转化生长因子-α（TGF-α）和细胞周期蛋白D1在头颈部VC、疣状增生（VH）和经典SCC中的表达。研究了26例VC、12例不同分化程度的SCC和4例VH。使用福尔马林固定、石蜡包埋的存档材料进行免疫组织化学（抗生物素蛋白-生物素免疫过氧化物酶技术），以研究癌基因及其肿瘤标志物的表达。在100%的VH、88%的VC和100%的SCC中发现了p53蛋白。在25%的VH、54%的VC、40%的高分化SCC（WDSCC）和100%的中低分化SCC（MDSCC/PDSCC）中观察到EGFR表达。在25%的VH、88%的VC、80%的WDSCC和100%的MDSCC/PDSCC中检测到TGF-α。在75%的VH、35%的VC、100%的WDSCC、67%的MDSCC和50%的PDSCC中观察到细胞周期蛋白D1表达。所有标志物的表达水平与这组鳞状病变的分级之间的相关性显示，p53和EGFR的相关系数具有统计学意义，而TGF-α和细胞周期蛋白D1则无统计学意义。
