Medical Faculty, Institute of Pathology, University of Ljubljana, Ljubljana, Slovenia.
Histopathology. 2012 Aug;61(2):257-65. doi: 10.1111/j.1365-2559.2012.04242.x. Epub 2012 Jun 13.
To investigate the expression of microRNAs miR-21, miR-31, miR-203, miR-125a-5p and miR-125b and proteins phosphatase and tensin homologue (PTEN) and p63 in verrucous carcinoma (VC) of the head and neck.
Thirty cases of VC, 50 cases of conventional squamous cell carcinoma (SCC) and 30 samples of normal epithelium of the head and neck were included. Real-time polymerase chain reaction and immunohistochemistry were used to analyse the expression of microRNAs and proteins, respectively. In comparison to normal epithelium, miR-21 was overexpressed in both VC and SCC and miR-31 was overexpressed in VC and in well- and moderately differentiated SCC. Levels of miR-203 were elevated in VC but unaltered or reduced in SCC, and levels of miR-125a-5p and miR-125b were reduced in VC but unaltered in SCC. PTEN was down-regulated in both VC and SCC, whereas p63 was down-regulated in VC but up-regulated in SCC. Differential expression of p63 in VC correlated inversely with the expression of miR-21 and miR-203.
Differences between VC, SCC and normal epithelium in expression profiles of investigated molecules indicate their association with the pathogenesis and clinicopathological characteristics of VC. Our results suggest that some microRNAs and proteins, particularly miR-125b, miR-203 and p63, might be useful in the diagnosis of VC.
研究微 RNA miR-21、miR-31、miR-203、miR-125a-5p 和 miR-125b 以及磷酸酶和张力蛋白同源物（PTEN）和 p63 在头颈部疣状癌（VC）中的表达。
纳入 30 例 VC、50 例常规鳞状细胞癌（SCC）和 30 例头颈部正常上皮组织。采用实时聚合酶链反应和免疫组织化学法分别分析微 RNA 和蛋白质的表达。与正常上皮组织相比，miR-21 在 VC 和 SCC 中均过表达，miR-31 在 VC 和分化良好及中度分化的 SCC 中过表达。miR-203 在 VC 中升高，但在 SCC 中不变或降低，miR-125a-5p 和 miR-125b 在 VC 中降低，但在 SCC 中不变。PTEN 在 VC 和 SCC 中均下调，而 p63 在 VC 中下调，在 SCC 中上调。VC 中 p63 的差异表达与 miR-21 和 miR-203 的表达呈负相关。
所研究分子在 VC、SCC 和正常上皮组织中的表达谱差异表明它们与 VC 的发病机制和临床病理特征有关。我们的结果表明，一些微 RNA 和蛋白质，特别是 miR-125b、miR-203 和 p63，可能有助于 VC 的诊断。
