Floyd R A, Soong L M, Culver P L
Cancer Res. 1976 Apr;36(4):1510-9.
Horseradish peroxidase and H2O2 mediate N-hydroxy-N-acetyl-2-aminofluorene (N-OH-AAF) conversion into two more potent carcinogens, 2-nitrosofluorene and N-acetoxy-N-acetyl-2-aminofluorene. Optical studies of this system indicate that horseradish peroxidase is operating as a peroxidase with N-OH-AAF as the electron donor. Our studies confirm the earlier finding that 2-nitrosofluorene and N-acetoxy-N-acetyl-2-aminofluorene are the products of the type II enzyme-mediated oxidation of N-OH-AAF, but surprisingly, the results with the type VI enzyme indicate that more 2-nitrosofluorene was formed and, in addition, another product absorbing at 245 nm was formed. If ascorbate is present in the N-OH-AAF/horseradish peroxidase/H2O2 system, ascorbate is oxidized preferentially. Cyanide, a known inhibitor of the peroxidase, does not inhibit when N-OH-AAF is the electron donor. The reaction products are the same in the presence or absence of cyanide.
辣根过氧化物酶和过氧化氢介导N-羟基-N-乙酰-2-氨基芴（N-OH-AAF）转化为另外两种更强效的致癌物，即2-亚硝基芴和N-乙酰氧基-N-乙酰-2-氨基芴。对该系统的光学研究表明，辣根过氧化物酶作为一种以N-OH-AAF为电子供体的过氧化物酶发挥作用。我们的研究证实了早期的发现，即2-亚硝基芴和N-乙酰氧基-N-乙酰-2-氨基芴是N-OH-AAF的II型酶介导氧化产物，但令人惊讶的是，VI型酶的实验结果表明生成了更多的2-亚硝基芴，此外，还形成了另一种在245nm处有吸收的产物。如果在N-OH-AAF/辣根过氧化物酶/过氧化氢系统中存在抗坏血酸，抗坏血酸会优先被氧化。氰化物是一种已知的过氧化物酶抑制剂，当N-OH-AAF作为电子供体时，它不会产生抑制作用。无论有无氰化物存在，反应产物都是相同的。
