[用于评估伤口愈合时间的大鼠损伤皮肤中EIIIA-纤连蛋白的表达]
[Expression of EIIIA-fibronectin in injured rat skin used in estimation of wound interval].
作者信息
Liu Ning-guo, Chen Yi-jiu, Huang Xiao-hua
机构信息
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063.
出版信息
Fa Yi Xue Za Zhi. 2002 Aug;18(3):129-31.
OBJECTIVE
To observe the means of fibronectin(FN) alternative splicing and the expression of EIIIA-FN variant in rat skin after bruise, for the sake of providing some help for forensic estimation of wound interval.
METHODS
Total RNA was isolated from wounded skin, and reverse transcription polymerase chain reaction was conducted to amplify target segments.
RESULTS
Detectable EIIIA+(526 bp) segments, lacked in normal organize, was amplified at 1 h after experimental wound, and the levels were increased within 24 h.
CONCLUSION
The alternative splicing EIIIA-fibronectin variant would be a satisfied criterion for research of skin injury.
目的
观察大鼠皮肤擦伤后纤连蛋白（FN）可变剪接方式及EIIIA - FN变异体的表达，为损伤时间的法医学鉴定提供依据。
方法
从损伤皮肤中提取总RNA，进行逆转录聚合酶链反应扩增目的片段。
结果
实验伤后1 h可扩增出正常组织中缺乏的可检测到的EIIIA +（526 bp）片段，且在24 h内其水平升高。
结论
EIIIA - 纤连蛋白变异体的可变剪接可作为皮肤损伤研究的良好指标。
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