Vitale A T, Pedroza-Seres M, Arrunategui-Correa V, Lee S J, DiMeo S, Foster C S, Colvin R B
Rhoads Molecular Immunology Laboratory Massachusetts Eye & Ear Infirmary, Boston 02114.
Invest Ophthalmol Vis Sci. 1994 Sep;35(10):3664-72.
The polymerase chain reaction was used to examine fibronectin (FN) expression during corneal scrape wounding with specific attention to the presence, absence, or gross changes of alternatively spliced FN as differentially expressed in the corneal stroma versus the epithelium in normal and wounded tissue.
Specific FN cDNA sequences were synthesized from rat cornea with total RNA and were amplified using various sets of synthetic oligonucleotide primers.
The authors observed the presence and sustained the expression of total FN, EIIIA, EIIIB, and V-region FN mRNA in normal and injured corneal stroma for up to 3 weeks after scrape wounding. In contrast, complementary overlying epithelial samples were virtually devoid of FN message.
These data suggest that functionally different, alternatively spliced FN isoforms may be involved both in the maintenance of the normal cornea and in wound healing, and that their synthesis occurs in situ principally by the stroma rather than by the epithelium.
运用聚合酶链反应检测角膜刮伤时纤连蛋白（FN）的表达情况，特别关注正常组织与受伤组织中角膜基质和上皮细胞中差异表达的可变剪接FN的存在、缺失或总体变化。
从大鼠角膜总RNA中合成特异性FN cDNA序列，并使用多组合成寡核苷酸引物进行扩增。
作者观察到，在刮伤后长达3周的时间里，正常和受伤的角膜基质中均存在并持续表达总FN、EIIIA、EIIIB和V区FN mRNA。相比之下，与之对应的上皮样本几乎没有FN信息。
这些数据表明，功能不同的可变剪接FN异构体可能参与正常角膜的维持和伤口愈合，并且它们的合成主要发生在基质原位，而非上皮细胞。
