来自印度尼西亚猫须草的一氧化氮抑制异海松烷型二萜类化合物。

Nitric oxide inhibitory isopimarane-type diterpenes from Orthosiphon stamineus of Indonesia.

作者信息

Awale Suresh, Tezuka Yasuhiro, Banskota Arjun H, Adnyana I Ketut, Kadota Shigetoshi

机构信息

Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630-Sugitani, Toyama 930-0194, Japan.

出版信息

J Nat Prod. 2003 Feb;66(2):255-8. doi: 10.1021/np020455x.

DOI:10.1021/np020455x

PMID:12608860

Abstract

A methanolic extract of Orthosiphon stamineus yielded six new highly oxygenated isopimarane-type diterpenes, orthosiphols U-Z (1-6), and 15 previously reported diterpenes. The isolated diterpenes all showed significant dose-dependent inhibitory effects on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophage-like J774.1 cells. Orthosiphols A (7), B (8), D (9), and X (4) showed more potent inhibitory activities than a positive control, N(G)-monomethyl-l-arginine (l-NMMA), and 1 displayed the strongest activity with an IC(50) value of 6.4 microM.

摘要

猫须草的甲醇提取物产生了六种新的高度氧化的异海松烷型二萜，即猫须草醇U-Z（1-6），以及15种先前报道的二萜。分离得到的二萜对脂多糖（LPS）激活的巨噬细胞样J774.1细胞中一氧化氮（NO）的产生均表现出显著的剂量依赖性抑制作用。猫须草醇A（7）、B（8）、D（9）和X（4）显示出比阳性对照N(G)-单甲基-L-精氨酸（L-NMMA）更强的抑制活性，化合物1表现出最强的活性，IC(50)值为6.4 microM。