台湾猫须草中的贝壳杉烷型和异海松烷型二萜及其一氧化氮抑制活性。

Staminane- and isopimarane-type diterpenes from Orthosiphon stamineus of Taiwan and their nitric oxide inhibitory activity.

作者信息

Nguyen Mai Thanh Thi, Awale Suresh, Tezuka Yasuhiro, Chien-Hsiung Chang, Kadota Shigetoshi

机构信息

Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630-Sugitani, Toyama 930-0194, Japan.

出版信息

J Nat Prod. 2004 Apr;67(4):654-8. doi: 10.1021/np030471+.

DOI:10.1021/np030471+

PMID:15104497

Abstract

From the MeOH extract of Taiwanese Orthosiphon stamineus, two new staminane-type diterpenes, staminols C (1) and D (2), and three new isopimarane-type diterpenes, orthosiphonone C (3) and D (4) and 14-deoxo-14-O-acetylorthosiphol Y (5), have been isolated together with 16 known diterpenes, orthosiphols A, B, D, K, M, N, O, X, and Y, nororthosiphonolide A, neoorthosiphol B, orthosiphonone A, secoorthosiphols B and C, 3-O-deacetylorthosiphol I, and 2-O-deacetylorthosiphol J. Their structures were determined on the basis of the spectroscopic data. All the newly isolated diterpenes exhibited dose-dependent inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophage-like J774.1 cells, and 2-O-deacetylorthosiphonone A showed the most potent activity, with an IC(50) value of 35.0 microM, comparable to that of the positive control N(G)-monomethyl-L-arginine (L-NMMA; IC(50), 35.7 microM).

摘要

从台湾猫须草的甲醇提取物中，分离得到了两种新的迷迭香烷型二萜，迷迭香醇C（1）和D（2），以及三种新的异海松烷型二萜，猫须草酮C（3）和D（4）以及14-脱氧-14-O-乙酰基猫须草素Y（5），同时还分离出16种已知的二萜，即猫须草醇A、B、D、K、M、N、O、X和Y、去甲猫须草内酯A、新猫须草醇B、猫须草酮A、裂猫须草醇B和C、3-O-去乙酰基猫须草醇I以及2-O-去乙酰基猫须草醇J。它们的结构是根据光谱数据确定的。所有新分离出的二萜在脂多糖（LPS）激活的巨噬细胞样J774.1细胞中均表现出对一氧化氮（NO）产生的剂量依赖性抑制作用，其中2-O-去乙酰基猫须草酮A活性最强，IC（50）值为35.0微摩尔，与阳性对照N（G）-单甲基-L-精氨酸（L-NMMA；IC（50），35.7微摩尔）相当。