Puthoff David P, Nettleton Dan, Rodermel Steven R, Baum Thomas J
Department of Plant Pathology, 351 Bessey Hall, Iowa State University, Ames, IA 50011, USA.
Plant J. 2003 Mar;33(5):911-21. doi: 10.1046/j.1365-313x.2003.01677.x.
With the availability of microarray technology, the expression profiles of thousands of genes can be monitored simultaneously to help determine the mechanisms of these biological processes. We conducted Affymetrix GeneChip microarray analyses of the Arabidopsis-cyst nematode interaction and employed a statistical procedure to analyze the resultant data, which allowed us to identify significant gene expression changes. Quantitative real-time RT-PCR assays were used to confirm the microarray analyses. The results of the expression profiling revealed 128 genes with altered steady-state mRNA levels following infection by the sugar beet cyst nematode (Heterodera schachtii; BCN), in contrast to only 12 genes that had altered expression following infection by the soybean cyst nematode (H. glycines; SCN). The expression of these 12 genes also changed following infection by BCN, i.e. we did not identify any genes regulated exclusively by SCN. The identification of 116 genes whose expression changes during successful cyst nematode parasitism by BCN suggests a potential involvement of these genes in the infection events starting with successful syncytium induction. Further characterization of these genes will permit the formulation of testable hypotheses to explain successful cyst nematode parasitism.
随着微阵列技术的出现，可以同时监测数千个基因的表达谱，以帮助确定这些生物学过程的机制。我们对拟南芥与孢囊线虫的相互作用进行了Affymetrix基因芯片微阵列分析，并采用统计程序分析所得数据，这使我们能够识别出显著的基因表达变化。定量实时RT-PCR分析用于确认微阵列分析结果。表达谱分析结果显示，感染甜菜孢囊线虫（Heterodera schachtii；BCN）后，有128个基因的稳态mRNA水平发生了变化，相比之下，感染大豆孢囊线虫（H. glycines；SCN）后只有12个基因的表达发生了变化。这12个基因的表达在感染BCN后也发生了变化，即我们没有鉴定出任何仅受SCN调控的基因。鉴定出116个在BCN成功寄生孢囊线虫期间表达发生变化的基因，表明这些基因可能参与了从成功诱导合胞体开始的感染事件。对这些基因的进一步表征将有助于形成可检验的假设，以解释孢囊线虫成功寄生的机制。
