高效液相色谱法分析骆驼蓬种子中去甲骆驼蓬碱、骆驼蓬醇、骆驼蓬碱和去氢骆驼蓬碱
HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.
作者信息
Kartal M, Altun M L, Kurucu S
机构信息
Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
出版信息
J Pharm Biomed Anal. 2003 Feb 26;31(2):263-9. doi: 10.1016/s0731-7085(02)00568-x.
A simple and sensitive method for separation and determination of harmol, harmalol, harmine and harmaline has been developed and validated. Harmol, harmalol, harmine and harmaline were separated using a Metasil ODS column by isocratic elution with flow rate 1.5 ml/min. The mobile phase composition was Isopropyl alcohol-Acetonitrile-Water-Formic acid (100:100:300:0.3) (v/v/v/v) and pH adjusted 8.6 with triethylamine. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for harmol, harmalol, harmine and harmaline were between 9.375-250, 30.750-246, 31.250-500 and 31.000-248 microg/ml, respectively. The method described was suitable for the determination of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.
已开发并验证了一种用于分离和测定去甲骆驼蓬碱、骆驼蓬醇、骆驼蓬碱和去氢骆驼蓬碱的简单灵敏方法。使用Metasil ODS柱，以1.5 ml/min的流速通过等度洗脱分离去甲骆驼蓬碱、骆驼蓬醇、骆驼蓬碱和去氢骆驼蓬碱。流动相组成为异丙醇 - 乙腈 - 水 - 甲酸（100:100:300:0.3）（v/v/v/v），并用三乙胺将pH调节至8.6。在330 nm处进行分光光度检测。去甲骆驼蓬碱、骆驼蓬醇、骆驼蓬碱和去氢骆驼蓬碱的线性检测范围分别为9.375 - 250、30.750 - 246、31.250 - 500和31.000 - 248 μg/ml。所述方法适用于测定骆驼蓬种子中的去甲骆驼蓬碱、骆驼蓬醇、骆驼蓬碱和去氢骆驼蓬碱。
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