Tascón Marcos, Benavente Fernando, Vizioli Nora M, Gagliardi Leonardo G
Laboratorio de Investigación y Desarrollo de Métodos Analíticos, LIDMA and División Química Analítica, Facultad de Ciencias Exactas, UNLP-CONICET, La PlataLaboratorio de Investigación y Desarrollo de Métodos Analíticos, LIDMA and División Química Analítica, Facultad de Ciencias Exactas, UNLP-CONICET, La Plata, B1900AJL, Argentina.
Departamento de Química Analítica, Instituto de Investigación en Nutrición y Seguridad Alimentaria, INSA-UB, Universidad de Barcelona, España.
Drug Test Anal. 2017 Apr;9(4):596-602. doi: 10.1002/dta.1989. Epub 2016 Jul 5.
The β-carboline alkaloids of the harmala (HAlks) group are compounds widely spread in many natural sources, but found at relatively high levels in some specific plants like Peganum harmala (Syrian rue) or Banisteriopsis caapi. HAlks are a reversible Mono Amino Oxidase type A Inhibitor (MAOI) and, as a consequence, these plants or their extracts can be used to produce psychotropic effects when are combined with psychotropic drugs based on amino groups. Since the occurrence and the levels of the HAlks in natural sources are subject to significant variability, more widespread use is not clinical but recreational or ritual, for example B. caapi is a known part of the Ayahuasca ritual mixture. The lack of simple methods to control the variable levels of these compounds in natural sources restricts the possibilities to dose in strict quantities and, as a consequence, limits its use with pharmacological or clinical purposes. In this work, we present a fast, simple, and robust method of quantifying simultaneously the six HAlks more frequently found in plants, i.e., harmine, harmaline, harmol, harmalol, harmane, and norharmane, by capillary electrophoresis instruments equipped with the more common detector UV. The method is applied to analyze these HAlks in P. Harmala seeds infusion which is a frequent intake form for these HAlks. The method is validated in three different instruments in order to evaluate the transferability and to compare the performances between them. In this case, harmaline, harmine, and harmol were found in the infusion samples. Copyright © 2016 John Wiley & Sons, Ltd.
骆驼蓬属的β-咔啉生物碱（HAlks）是一类广泛存在于多种天然来源中的化合物，但在某些特定植物中含量相对较高，如骆驼蓬（叙利亚芸香）或卡皮木。HAlks是一种可逆的A型单胺氧化酶抑制剂（MAOI），因此，当这些植物或其提取物与基于氨基的精神药物合用时，可产生精神效应。由于天然来源中HAlks的含量和水平存在显著差异，其更多地被用于娱乐或仪式用途而非临床，例如卡皮木是著名的死藤水仪式混合物的一部分。缺乏控制天然来源中这些化合物可变水平的简单方法限制了精确给药的可能性，因此也限制了其在药理学或临床方面的应用。在这项工作中，我们提出了一种快速、简单且可靠的方法，可通过配备常见紫外检测器的毛细管电泳仪同时定量植物中更常见的六种HAlks，即去氢骆驼蓬碱、骆驼蓬碱、去甲氧基骆驼蓬碱、去氢骆驼蓬醇、哈尔满和去甲哈尔满。该方法用于分析骆驼蓬种子浸液中的这些HAlks，骆驼蓬种子浸液是这些HAlks常见的摄入形式。该方法在三种不同仪器上进行了验证，以评估其可转移性并比较它们之间的性能。在这种情况下，浸液样品中检测到了骆驼蓬碱、去氢骆驼蓬碱和去甲氧基骆驼蓬碱。版权所有© 2016约翰·威利父子有限公司。
