1型人类免疫缺陷病毒在正常人口腔角质形成细胞中的感染与复制。
Human immunodeficiency virus type 1 infection and replication in normal human oral keratinocytes.
作者信息
Liu Xuan, Zha Junli, Chen Hongying, Nishitani Junko, Camargo Paulo, Cole Steve W, Zack Jerome A
机构信息
Department of Oral and Maxillofacial Surgery, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
出版信息
J Virol. 2003 Mar;77(6):3470-6. doi: 10.1128/jvi.77.6.3470-3476.2003.
Recent epidemiologic studies show increasing human immunodeficiency virus type 1 (HIV-1) transmission through oral-genital contact. This paper examines the possibility that normal human oral keratinocytes (NHOKs) might be directly infected by HIV or might convey infectious HIV virions to adjacent leukocytes. PCR analysis of proviral DNA constructs showed that NHOKs can be infected by CXCR4-tropic (NL4-3 and ELI) and dualtropic (89.6) strains of HIV-1 to generate a weak but productive infection. CCR5-tropic strain Ba-L sustained minimal viral replication. Antibody inhibition studies showed that infection by CXCR4-tropic viral strains is mediated by the galactosylceramide receptor and the CXCR4 chemokine coreceptor. Coculture studies showed that infectious HIV-1 virions can also be conveyed from NHOKs to activated peripheral blood lymphocytes, suggesting a potential role of oral epithelial cells in the transmission of HIV infection.
近期的流行病学研究表明，通过口交传播的人类免疫缺陷病毒1型（HIV-1）感染呈上升趋势。本文探讨了正常人口腔角质形成细胞（NHOKs）可能被HIV直接感染或可能将感染性HIV病毒颗粒传递给相邻白细胞的可能性。对前病毒DNA构建体的PCR分析表明，NHOKs可被HIV-1的CXCR4嗜性毒株（NL4-3和ELI）和双嗜性毒株（89.6）感染，从而产生弱但有生产性的感染。CCR5嗜性毒株Ba-L的病毒复制极少。抗体抑制研究表明，CXCR4嗜性病毒株的感染是由半乳糖神经酰胺受体和CXCR4趋化因子共受体介导的。共培养研究表明，感染性HIV-1病毒颗粒也可从NHOKs传递至活化的外周血淋巴细胞，提示口腔上皮细胞在HIV感染传播中可能发挥作用。
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