来自成人皮肤的角质形成细胞培养物中的干细胞（p63(+)）
Stem cells (p63(+)) in keratinocyte cultures from human adult skin.
作者信息
Radu E, Simionescu Olga, Regalia T, Dumitrescu Doina, Popescu L M
机构信息
Department of Cellular and Molecular Medicine, Faculty of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
出版信息
J Cell Mol Med. 2002 Oct-Dec;6(4):593-8. doi: 10.1111/j.1582-4934.2002.tb00457.x.
Abstract
Epidermal stem cells (ESC) are responsible for maintaining skin cellular homeostasis, as they give rise to fast-dividing transit amplifying cells committed to terminal differentiation, while retaining their self-renewal capacity. However, no pure ESC cultures are available and no highly specific cytochemical marker was identified. We report here the experimental conditions allowing the selective enrichment in ESC, using cultured adult human keratinocytes. The main step was the selection of cells able to rapidly adhere to human collagen type IV in vitro. Thus, an increased proportion of putative ESC of about 65% was obtained, as demonstrated by p63 expression.
摘要
表皮干细胞（ESC）负责维持皮肤细胞的稳态，因为它们会产生快速分裂的过渡增殖细胞，这些细胞致力于终末分化，同时保留自我更新能力。然而，目前尚无纯ESC培养物，也未鉴定出高度特异性的细胞化学标志物。我们在此报告了利用培养的成人人类角质形成细胞实现ESC选择性富集的实验条件。主要步骤是选择能够在体外快速黏附于IV型人胶原蛋白的细胞。因此，如p63表达所示，获得了约65%的假定ESC比例增加。
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