Jones P H, Watt F M
Keratinocyte Laboratory, Imperial Cancer Research Fund, London, England.
Cell. 1993 May 21;73(4):713-24. doi: 10.1016/0092-8674(93)90251-k.
The epidermis is believed to contain two types of proliferating cells: stem cells and cells with a lower capacity for self-renewal and higher probability of undergoing terminal differentiation (transit amplifying cells). We report that keratinocytes with characteristics of stem cells can be isolated from cultured human epidermis on the basis of high surface expression of beta 1 integrins and rapid adhesion to extracellular matrix (ECM) proteins. Among keratinocytes there was a log linear relationship between the relative level of beta 1 integrins on the cell surface and proliferative capacity; furthermore, the cells with the highest colony-forming efficiency adhered most rapidly to type IV collagen, fibronectin, or keratinocyte ECM. Proliferating keratinocytes that adhered more slowly had characteristics of transit amplifying cells: after one to five rounds of division, all of their daughters underwent terminal differentiation. Since stem cells can be isolated to greater than 90% purity on the basis of their adhesive properties, it will now be possible to investigate the mechanisms that regulate the fate of their progeny.
干细胞以及自我更新能力较低且发生终末分化可能性较高的细胞（过渡放大细胞）。我们报告称，具有干细胞特征的角质形成细胞可基于β1整合素的高表面表达以及对细胞外基质（ECM）蛋白的快速黏附，从培养的人表皮中分离出来。在角质形成细胞中，细胞表面β1整合素的相对水平与增殖能力之间存在对数线性关系；此外，集落形成效率最高的细胞对IV型胶原、纤连蛋白或角质形成细胞ECM的黏附最为迅速。黏附较慢的增殖角质形成细胞具有过渡放大细胞的特征：经过一到五轮分裂后，它们所有的子代都会发生终末分化。由于基于其黏附特性可将干细胞分离至纯度超过90%，现在将有可能研究调控其子代命运的机制。
