Vogel H G
Connect Tissue Res. 1975;3(4):237-44. doi: 10.3109/03008207509152184.
After oral treatment with D-penicillamine (D-Pc) or with aminoacetonitrile (AAn) for 10 days, mechanical and chemical parameters were studied simultaneously in various organs of Sprague Dawley rats. Tensile strength of skin strips and of tail tendons, breaking strength of femur bones and tensile strength of granuloma tissue (induced by implanted glass rods) were measured and calculated. In the same tissue the soluble collagen fractions and the insoluble collagen were determined. Total collagen and the ratio insoluble vs. soluble collagen were calculated. Tensile strength of skin, tendon and granuloma tissue were greatly reduced by D-Pc treatment but only minimally influenced by AAN treatment. On the other hand only AAN significantly reduced the breaking strength of bone. All these changes were closely correlated with the content of insoluble collagen in the respective tissues. The correlation coefficients to total collagen were similar but lower. The correlation coefficients between strength and the ratio insoluble vs. soluble collagen were generally still lower. Earlier findings in aged and corticoid treated rats, proving that insoluble collagen content determines mechanical strength of connnective and supporting tissue thus could be confirmed.
用D-青霉胺（D-Pc）或氨基乙腈（AAn）对Sprague Dawley大鼠进行口服治疗10天后，同时研究了大鼠各器官的力学和化学参数。测量并计算了皮肤条带和尾腱的抗张强度、股骨的断裂强度以及肉芽肿组织（由植入玻璃棒诱导）的抗张强度。在相同组织中测定了可溶性胶原组分和不溶性胶原。计算了总胶原以及不溶性胶原与可溶性胶原的比例。D-Pc处理显著降低了皮肤、肌腱和肉芽肿组织的抗张强度，但AAN处理对其影响极小。另一方面，只有AAN显著降低了骨的断裂强度。所有这些变化都与相应组织中不溶性胶原的含量密切相关。与总胶原的相关系数相似但较低。强度与不溶性胶原与可溶性胶原比例之间的相关系数通常更低。在老年和用皮质激素处理的大鼠中早期发现，证明不溶性胶原含量决定结缔组织和支持组织的力学强度，这一点在此得到了证实。
