Garcia C V, Breier A R, Steppe M, Schapoval E E S, Oppe T P
Departamento de Produção e Controle de Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, 90610-000, RS, Brazil.
J Pharm Biomed Anal. 2003 Mar 10;31(3):597-600. doi: 10.1016/s0731-7085(02)00695-7.
The aim of this research was to validate a high performance liquid chromatographic method for the quantitative determination of dexamethasone acetate contained in cream preparation. A MetaSil octadecyl silane (250 x 4.6 mm, 5 microm) column, a methanol: water (65:35; v/v) mobile phase (1.0 ml min(-1)) and an UV detector (set at 254 nm) were used to evaluate the parameters: linearity, precision, accuracy, specificity, as well as, quantitation and detection limits. The calibration curve showed a correlation coefficient of 0.9999. The precision was demonstrated by the relative standard deviation (RSD) of 0.53. The recovery test resulted in an average of 97.85%, what confirmed the accuracy of the method. The quantitation and detection limits determined were 1.41 and 0.47 microg ml(-1), respectively. The specificity test showed there was no interference in the drug peak.
