Sagirli Olcay, Onal Armağan, Toker Sidika, Oztunç Aysel
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34116 Istanbul, Turkey.
J AOAC Int. 2009 Nov-Dec;92(6):1658-62.
A rapid and simple HPLC method was developed for the determination of linezolid (LNZ) in human breast milk after a simple protein precipitation with methanol. The chromatographic separation was achieved on a C18 column (5 microm, 250 x 4.6 mm id) using a mobile phase of acetonitrile-10 mM acetic acid (25:75, v/v) at a flow rate of 1 mL/min. The LNZ peak was measured by photodiode array detection at 250 nm. The calibration graph was linear over the range of 0.5-20.0 microg/mL. The limits of detection and quantitation were found to be 0.1 and 0.5 microg/mL, respectively. The precision of the assay and the recovery of LNZ from breast milk at three different concentrations were assessed. The intraday and interday RSD values were found to be < 5%. The mean absolute recovery was 85.33%. The developed method was successfully applied to the determination of LNZ in breast milk obtained from the breastfeeding mother after oral administration of LNZ.
建立了一种快速简便的高效液相色谱法，用于测定人母乳中的利奈唑胺（LNZ），该方法先用甲醇进行简单的蛋白沉淀。色谱分离在C18柱（5微米，250×4.6毫米内径）上进行，流动相为乙腈-10 mM乙酸（25:75，v/v），流速为1 mL/分钟。利奈唑胺峰通过在250 nm处的光电二极管阵列检测进行测量。校准曲线在0.5-20.0微克/毫升范围内呈线性。检测限和定量限分别为0.1和0.5微克/毫升。评估了该测定方法的精密度以及在三种不同浓度下利奈唑胺从母乳中的回收率。日内和日间相对标准偏差值均<5%。平均绝对回收率为85.33%。所建立的方法成功应用于口服利奈唑胺后从母乳喂养母亲获得的母乳中利奈唑胺的测定。
