Schmidt Sven, Müller Rainer H
Pharmazeutische Technologie, Biopharmazie und Biotechnologie, Institut für Pharmazie, Freie Universität Berlin, Kelchstrasse 31, D-12169 Berlin, Germany.
Int J Pharm. 2003 Mar 18;254(1):3-5. doi: 10.1016/s0378-5173(02)00667-1.
Nephrotoxicity of the conventional Amphotericin B formulation Fungizone is the most common side effect in treatment of systemic fungal infections. Lipid formulations of Amphotericin B including fat emulsions showed a reduced nephrotoxicity. In vivo distribution studies of lipid formulations have shown an accumulation of Amphotericin B in liver and spleen, while concentration in the kidneys is reduced. Blood proteins adsorbed onto particles after intravenous administration are regarded as the key factors determining their in vivo fate. Two-dimensional polyacrylamid gel electrophoresis is a powerful tool for analysis of protein adsorption patterns. This paper deals with the question if there is any correlation between proteins adsorbed on surfaces of AmB fat emulsions produced with a new production technique and the potentially organ distribution of this formulation.
传统两性霉素B制剂（两性霉素B去氧胆酸盐）的肾毒性是治疗全身性真菌感染时最常见的副作用。包括脂肪乳剂在内的两性霉素B脂质制剂显示出肾毒性降低。脂质制剂的体内分布研究表明，两性霉素B在肝脏和脾脏中蓄积，而在肾脏中的浓度降低。静脉给药后吸附在颗粒上的血液蛋白质被认为是决定其体内命运的关键因素。二维聚丙烯酰胺凝胶电泳是分析蛋白质吸附模式的有力工具。本文探讨了采用新生产技术生产的两性霉素B脂肪乳剂表面吸附的蛋白质与该制剂潜在的器官分布之间是否存在任何相关性的问题。
