Bekman Evguenia, Henrique Domingos
Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal.
Gene Expr Patterns. 2002 Nov;2(1-2):73-7. doi: 10.1016/s0925-4773(02)00379-9.
The Drosophila melanogaster gene prickle-spiny-legs (pk) functions in an intercellular feedback loop that is central to the establishment of planar cell polarity in the eye and epidermis of the fly, by modulating Frizzled-Disheveled signalling. Here we identify three mouse prickle-related genes (dyxin, testin and prickle) and describe their expression pattern during murine embryogenesis (E7.5-E15.5). We report that the three genes are expressed in restricted areas of the developing mouse brain: dyxin in the most ventral region of the neural tube and in some localized regions of the ventricular layer of the mesencephalon and rhombencephalon, prickle in the pons region, ventrolateral part of rhombencephalon and motoneurons in the spinal cord, and testin in differentiating neurons of the spinal cord and retina. At the stages analyzed, the main site of expression of testin is the migrating cranial neural crest, while the expression of dyxin is noticeable in myotomal cells and its derivatives, with prickle expression being reciprocally localized to some sclerotomal derivatives, like bone primordia. prickle is also expressed in the apical ectodermal ridge and the most distal mesenchyme of the forming limb buds.
果蝇基因“刺棘腿”（pk）在一个细胞间反馈回路中发挥作用，该回路通过调节卷曲蛋白-散乱蛋白信号传导，对果蝇眼睛和表皮平面细胞极性的建立至关重要。在此，我们鉴定出三个与小鼠刺棘相关的基因（dyxin、testin和prickle），并描述它们在小鼠胚胎发育过程（E7.5-E15.5）中的表达模式。我们报告称，这三个基因在发育中的小鼠脑的特定区域表达：dyxin在神经管最腹侧区域以及中脑和后脑室层的一些局部区域表达，prickle在脑桥区域、后脑腹外侧部分以及脊髓运动神经元中表达，testin在脊髓和视网膜的分化神经元中表达。在所分析的阶段，testin的主要表达部位是迁移的颅神经嵴，而dyxin在肌节细胞及其衍生物中有明显表达，prickle的表达则相反地定位于一些硬骨节衍生物，如骨原基。prickle也在顶端外胚层嵴和形成肢芽的最远端间充质中表达。
