Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal.
Mech Dev. 2002 Dec;119 Suppl 1:S77-81. doi: 10.1016/s0925-4773(03)00095-9.
The Drosophila melanogaster gene prickle-spiny-legs (pk) functions in an intercellular feedback loop that is central to the establishment of planar cell polarity in the eye and epidermis of the fly, by modulating Frizzled-Disheveled signalling. Here we identify three mouse prickle-related genes (dyxin, testin and prickle) and describe their expression pattern during murine embryogenesis (E7.5-E15.5). We report that the three genes are expressed in restricted areas of the developing mouse brain: dyxin in the most ventral region of the neural tube and in some localized regions of the ventricular layer of the mesencephalon and rhombencephalon, prickle in the pons region, ventrolateral part of rhombencephalon and motoneurons in the spinal cord, and testin in differentiating neurons of the spinal cord and retina. At the stages analyzed, the main site of expression of testin is the migrating cranial neural crest, while the expression of dyxin is noticeable in myotomal cells and its derivatives, with prickle expression being reciprocally localized to some sclerotomal derivatives, like bone primordia. prickle is also expressed in the apical ectodermal ridge and the most distal mesenchyme of the forming limb buds.
果蝇 melanogaster 基因 prickle-spiny-legs（pk）在细胞间反馈回路中发挥作用，该回路是果蝇眼睛和表皮中平面细胞极性建立的核心，通过调节 Frizzled-Disheveled 信号。在这里，我们鉴定了三个小鼠 prickle 相关基因（dyxin、testin 和 prickle），并描述了它们在小鼠胚胎发生过程中的表达模式（E7.5-E15.5）。我们报告说，这三个基因在发育中的小鼠大脑的特定区域表达：dyxin 在神经管的最腹侧区域以及中脑和后脑的室管膜层的一些局部区域，prickle 在脑桥区域、后脑的腹外侧部分和脊髓中的运动神经元，testin 在脊髓和视网膜的分化神经元中表达。在分析的阶段，testin 的主要表达部位是迁移的颅神经嵴，而 dyxin 的表达在肌节细胞及其衍生物中很明显，prickle 的表达则与一些软骨原基等软骨组织衍生物相互定位。prickle 还在形成的肢芽的顶端外胚层嵴和最远端的间质中表达。
