Sauka-Spengler Tatjana, Le Mentec Chantal, Lepage Mario, Mazan Sylvie
Equipe Développement et Evolution des Vertébrés, UPRES-A 8080, Université Paris- Sud, 91405 Orsay Cedex, France.
Gene Expr Patterns. 2002 Nov;2(1-2):99-103. doi: 10.1016/s0925-4773(02)00301-5.
We report the embryonic expression in the lamprey Lampetra fluviatilis of Tbx1, the main candidate gene involved in DiGeorge/velo-cardio-facial syndrome (DGS/VCFS). From the end of neurulation to stage 26, Tbx1 becomes progressively expressed in all developing pharyngeal arches, as they form. Transcripts are mainly restricted to the mesodermal core and to the posterior pharyngeal endoderm, excluding ingressing neural crest cells. They are also present in the otic vesicle, in a ventral and posterior location. From a later stage (stage 27) onwards, additional expression domains in the head mesenchyme, later contributing to labial muscle precursors, and in the cloacal region, become visible. The comparison of these data with those reported in the chick and the mouse indicates a high conservation of Tbx1 expression in the pharyngeal arches among vertebrates.
我们报告了七鳃鳗（Lampetra fluviatilis）中Tbx1基因的胚胎表达情况，Tbx1是与DiGeorge/心-面-腭裂综合征（DGS/VCFS）相关的主要候选基因。从神经胚形成末期到第26阶段，随着所有发育中的咽弓形成，Tbx1逐渐在其中表达。转录本主要局限于中胚层核心和咽后内胚层，不包括侵入的神经嵴细胞。它们也存在于耳囊中，位于腹侧和后侧。从后期阶段（第27阶段）开始，在头部间充质（后来形成唇肌前体）和泄殖腔区域中出现了额外的表达域。将这些数据与在鸡和小鼠中报道的数据进行比较，表明脊椎动物咽弓中Tbx1表达具有高度保守性。
