Autologous cultured mucosal graft to cover large intraoral mucosal defects: a clinical study.

PURPOSE

The study evaluated the performance of cultured mucosal grafts (CMG) for large intraoral mucosal defects caused by surgical excision of mucosal pathology.

PATIENTS AND METHODS

Eleven patients (10 men and 1 woman; mean age, 52.4 +/- 14.1 years) were treated using CMG following mucosal defects. A biopsy specimen (0.2 to 0.5 cm(2)) was taken from a clinically healthy oral mucosa a few weeks before the surgery. Mucosal epithelial cells were cultured in vitro over a feeder layer of fibroblasts. Usually, within 3 to 4 weeks, multilayered sheets (50 to 250 cm(2)) were generated. The cultured sheets were placed on the mucosal defects (48.4 +/- 21.7 cm(2); 8 to 70 cm(2)) and anchored to the adjacent tissue with sutures.

RESULTS

One week after surgery, the CMG survived and adhered to the wound bed. Three weeks postoperatively, the grafted site was smooth and keratinized, without infection or scar contraction. Three months postoperatively, the grafted area was covered with a healthy mucosa, indistinguishable from the adjacent mucosa.

CONCLUSION

CMG is a useful grafting material for large intraoral mucosal defects.