Bodner Lipa, Grossman Nili
Department of Oral and Maxillofacial Surgery, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel.
J Oral Maxillofac Surg. 2003 Feb;61(2):169-73. doi: 10.1053/joms.2003.50043.
The study evaluated the performance of cultured mucosal grafts (CMG) for large intraoral mucosal defects caused by surgical excision of mucosal pathology.
Eleven patients (10 men and 1 woman; mean age, 52.4 +/- 14.1 years) were treated using CMG following mucosal defects. A biopsy specimen (0.2 to 0.5 cm(2)) was taken from a clinically healthy oral mucosa a few weeks before the surgery. Mucosal epithelial cells were cultured in vitro over a feeder layer of fibroblasts. Usually, within 3 to 4 weeks, multilayered sheets (50 to 250 cm(2)) were generated. The cultured sheets were placed on the mucosal defects (48.4 +/- 21.7 cm(2); 8 to 70 cm(2)) and anchored to the adjacent tissue with sutures.
One week after surgery, the CMG survived and adhered to the wound bed. Three weeks postoperatively, the grafted site was smooth and keratinized, without infection or scar contraction. Three months postoperatively, the grafted area was covered with a healthy mucosa, indistinguishable from the adjacent mucosa.
CMG is a useful grafting material for large intraoral mucosal defects.
本研究评估了培养黏膜移植物（CMG）在治疗因手术切除黏膜病变导致的口腔内大面积黏膜缺损方面的性能。
11例患者（10名男性和1名女性；平均年龄52.4±14.1岁）在出现黏膜缺损后接受了CMG治疗。在手术前几周，从临床健康的口腔黏膜取一块活检标本（0.2至0.5平方厘米）。黏膜上皮细胞在成纤维细胞饲养层上进行体外培养。通常在3至4周内，可生成多层薄片（50至250平方厘米）。将培养的薄片置于黏膜缺损处（48.4±21.7平方厘米；8至70平方厘米），并用缝线固定于相邻组织。
术后1周，CMG存活并附着于创面床。术后3周，移植部位平整且角化，无感染或瘢痕挛缩。术后3个月，移植区域被健康黏膜覆盖，与相邻黏膜无明显差异。
CMG是治疗口腔内大面积黏膜缺损的一种有用的移植材料。
