Cho Sung-Yeon, Park Sang-Joon, Kwon Myung-Ja, Jeong Tae-Sook, Bok Song-Hae, Choi Woo-Young, Jeong Won-Il, Ryu Si-Yun, Do Sun-Hee, Lee Cha-Soo, Song Jae-Chan, Jeong Kyu-Shik
Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon City, South Korea.
Mol Cell Biochem. 2003 Jan;243(1-2):153-60. doi: 10.1023/a:1021624520740.
Quercetin is a flavonoid molecule ubiquitous in nature and functions as an anti-oxidant and anti-inflammatory agent with little toxicity in vivo and in vitro. Dose- and time-dependent effect of quercetin has been investigated on proinflammatory cytokine expression and NO production, focusing on its effects on the MAP kinases and the NF-kappaB signal transduction pathways in LPS-stimulated RAW 264.7 cells by using RT-PCR and immunoblotting. Quercetin strongly reduced activation of phosphorylated ERK kinase and p38 MAP kinase but not JNK MAP kinase by LPS treatment. In addition, quercetin treatment inhibited NF-kappaB activation through stabilization of the NF-kappaB/IkappaB complex and IkappaB degradation and proinflammatory cytokines and NO/iNOS expression. Quercetin may exert its anti-inflammatory and immunomodulatory properties in the effect molecules such as proinflammatory cytokines and NO/iNOS by suppressing the activation of ERK and p38 MAP kinase, and NF-kappaB/IkappaB signal transduction pathways.
槲皮素是一种在自然界中普遍存在的类黄酮分子，具有抗氧化和抗炎作用，在体内和体外的毒性都很小。通过逆转录聚合酶链反应（RT-PCR）和免疫印迹法，研究了槲皮素对促炎细胞因子表达和一氧化氮（NO）生成的剂量和时间依赖性影响，重点关注其对脂多糖（LPS）刺激的RAW 264.7细胞中丝裂原活化蛋白激酶（MAP激酶）和核因子κB（NF-κB）信号转导途径的影响。通过LPS处理，槲皮素强烈降低了磷酸化细胞外信号调节激酶（ERK激酶）和p38 MAP激酶的激活，但对c-Jun氨基末端激酶（JNK MAP激酶）没有影响。此外，槲皮素处理通过稳定NF-κB/IκB复合物、抑制IκB降解以及促炎细胞因子和NO/诱导型一氧化氮合酶（iNOS）的表达，抑制了NF-κB的激活。槲皮素可能通过抑制ERK和p38 MAP激酶以及NF-κB/IκB信号转导途径的激活，在促炎细胞因子和NO/iNOS等效应分子中发挥其抗炎和免疫调节特性。
