Danilov Alexandre I, Andersson Magnus, Bavand Nasrin, Wiklund N Peter, Olsson Tomas, Brundin Lou
Department of Clinical Neuroscience, Karolinska Institutet, Karolinska Hospital, S-17176 Stockholm, Sweden.
J Neuroimmunol. 2003 Mar;136(1-2):112-8. doi: 10.1016/s0165-5728(02)00464-2.
Nitric oxide (NO) is formed as a consequence of induction of the iNOS enzyme during inflammatory disorders. To investigate NO production in multiple sclerosis (MS), we determined the concentrations of its oxidation products (NOx) in the cerebrospinal fluid (CSF) and plasma of 61 MS patients. The patients were divided into three groups on the basis of their clinical disease activity. The total levels of NOx in CSF were significantly increased in all MS groups as compared to healthy controls and tension headache patients. CSF nitrite correlated with clinical disease activity. At exacerbation, the CSF nitrite levels exceed the plasma level. This suggests that clinical disease activity is due to a CNS inflammatory response, which is more intense and qualitatively different from that during clinical stable phases. This study supports NO involvement in the pathogenesis of MS and determination of nitrite levels may be useful a surrogate marker for disease activity.
一氧化氮（NO）是在炎症性疾病期间诱导诱导型一氧化氮合酶（iNOS）而形成的。为了研究多发性硬化症（MS）中NO的产生情况，我们测定了61例MS患者脑脊液（CSF）和血浆中其氧化产物（NOx）的浓度。根据临床疾病活动度将患者分为三组。与健康对照组和紧张性头痛患者相比，所有MS组CSF中NOx的总水平均显著升高。CSF亚硝酸盐与临床疾病活动度相关。病情加重时，CSF亚硝酸盐水平超过血浆水平。这表明临床疾病活动是由于中枢神经系统（CNS）炎症反应所致，该反应比临床稳定期更强烈且在性质上有所不同。本研究支持NO参与MS的发病机制，并且测定亚硝酸盐水平可能是疾病活动度的有用替代标志物。
