Cross A H, Manning P T, Keeling R M, Schmidt R E, Misko T P
Department of Neurology and Neurosurgery, Washington University School of Medicine, St. Louis, MO 63110, USA.
J Neuroimmunol. 1998 Aug 1;88(1-2):45-56. doi: 10.1016/s0165-5728(98)00078-2.
Peroxynitrite, generated by the reaction of nitric oxide (NO) with superoxide at sites of inflammation, is a strong oxidant capable of damaging tissues and cells. Detection of nitrotyrosine (NT) at inflammatory sites serves as a biochemical marker for peroxynitrite-mediated damage. In this study, NT was detected immunohistochemically within autopsied CNS tissues from six of nine multiple sclerosis (MS) patients, and in most of the MS sections displaying inflammation. Nitrite and nitrate, the stable oxidation products of NO and peroxynitrite, respectively, were measured in cerebrospinal fluid samples obtained from MS patients and controls. Levels of nitrate were elevated significantly during clinical relapses of MS. These data suggest that peroxynitrite formation is a major consequence of NO produced in MS-affected CNS and implicate a role for this powerful oxidant in the pathogenesis of MS.
过氧亚硝酸盐由一氧化氮（NO）与炎症部位的超氧化物反应生成，是一种能够损伤组织和细胞的强氧化剂。在炎症部位检测硝基酪氨酸（NT）可作为过氧亚硝酸盐介导损伤的生化标志物。在本研究中，通过免疫组织化学方法在9例多发性硬化症（MS）患者中的6例尸检中枢神经系统组织中检测到了NT，并且在大多数显示炎症的MS切片中也检测到了NT。分别测量了从MS患者和对照组获得的脑脊液样本中NO和过氧亚硝酸盐的稳定氧化产物亚硝酸盐和硝酸盐。在MS临床复发期间，硝酸盐水平显著升高。这些数据表明，过氧亚硝酸盐的形成是MS影响的中枢神经系统中产生的NO的主要后果，并提示这种强氧化剂在MS发病机制中起作用。
