Arias Y Meriah, Tebo Bradley M
Marine Biology Research Division and Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, CA 92093-0202, USA.
Appl Environ Microbiol. 2003 Mar;69(3):1847-53. doi: 10.1128/AEM.69.3.1847-1853.2003.
In time course experiments, bacterial community compositions were compared between a sulfidogenic and two nonsulfidogenic Cr(VI)-reducing consortia enriched from metal-contaminated sediments. The consortia were subjected to 0 and 0.85 mM or 1.35 mM Cr(VI), and Cr(VI) reduction, growth, and denaturing gradient gel electrophoresis profiles of PCR products of small-subunit (16S) ribosomal genes were compared. Results showed that although Cr(VI) was completely reduced by the three consortia, Cr(VI) inhibited cell growth, with sulfate-reducing bacteria being particularly sensitive to Cr(VI) toxicity relative to other bacteria in the consortia.
在时间进程实验中,比较了从金属污染沉积物中富集的一个产硫化物和两个不产硫化物的六价铬还原菌群的细菌群落组成。这些菌群分别暴露于0和0.85 mM或1.35 mM的六价铬环境中,并比较了六价铬还原情况、生长情况以及小亚基(16S)核糖体基因PCR产物的变性梯度凝胶电泳图谱。结果表明,尽管三个菌群都能将六价铬完全还原,但六价铬抑制了细胞生长,相对于菌群中的其他细菌,硫酸盐还原菌对六价铬毒性尤为敏感。