Wang Gang, Chen Qiming, Zhu Yan, Ke Daoping, Qian Weidong, Song Wenying
Laboratory of Neurophysiology, Department of Physiology, Anhui Medical University, Hefei 230032, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2002 Dec;25(12):732-5.
To evaluate the value of specific neuronal anti-Hu antibody in the early diagnosis of small cell lung cancer (SCLC).
Ninety-eight patients with SCLC were included in the study. Specific neuronal anti-Hu antibody was detected by immunohistochemistry (the ABC method) and Western blot.
The sera from 40% (32/80) of the patients in group A (without paraneoplastic syndrome of the nervous system) reacted with the frozen sections of human cerebra and were stained by indirect immunoperoxidase method at final dilutions of 1:1,000 to 1:8,000, while the sera from 89% of the patients (16/18) in group B (with paraneoplastic syndrome of the nervous system) reacted at final dilutions of 1:1,000 to 1:64,000. Western blot analysis revealed that the specific neuronal anti-Hu antibody was identified at several bands of approximately 35,000 approximately 40,000 with nuclear extracts, and at 40,000 with HuD clonal protein. The anti-Hu antibody was detected in only two patients from group A, but in 16 patients from group B. The antibody was not detected in sera from 32 normal human subjects.
Specific neuronal anti-Hu antibody at high titers was detected in patients with SCLC not complicated with paraneoplastic syndrome of the nervous system. This antibody may be useful in the early diagnosis of SCLC.
评估特异性神经元抗Hu抗体在小细胞肺癌（SCLC）早期诊断中的价值。
98例SCLC患者纳入本研究。采用免疫组织化学（ABC法）和蛋白质印迹法检测特异性神经元抗Hu抗体。
A组（无神经系统副肿瘤综合征）40%（32/80）患者的血清与人脑冰冻切片发生反应，间接免疫过氧化物酶法染色的终末稀释度为1:1000至1:8000，而B组（有神经系统副肿瘤综合征）89%（16/18）患者的血清在终末稀释度为1:1000至1:64000时发生反应。蛋白质印迹分析显示，用核提取物在约35000至约40000的几条条带处鉴定出特异性神经元抗Hu抗体，用HuD克隆蛋白在40000处鉴定出该抗体。A组仅2例患者检测到抗Hu抗体，而B组16例患者检测到该抗体。32名正常受试者的血清中未检测到该抗体。
在无神经系统副肿瘤综合征的SCLC患者中检测到高滴度的特异性神经元抗Hu抗体。该抗体可能有助于SCLC的早期诊断。
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