Dalmau J, Furneaux H M, Gralla R J, Kris M G, Posner J B
Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Ann Neurol. 1990 May;27(5):544-52. doi: 10.1002/ana.410270515.
We looked for the presence of the anti-Hu antibody in the sera from 50 normal subjects; 44 patients with small cell lung cancer, not associated with paraneoplastic disease; and 25 patients with small cell lung cancer associated with paraneoplastic sensory neuropathy, encephalomyelitis, or both. Using the avidin-biotin immunoperoxidase method and a highly sensitive quantitative Western blot analysis, the anti-Hu antibody was not detected in the 50 normal human sera. Seven of the 44 patients with small cell lung cancer but no paraneoplastic syndrome had detectable levels (average titer, 76 U/ml) of anti-Hu antibody on Western blot. These levels are significantly lower than the average titer of the 25 patients who had small cell lung cancer and paraneoplastic sensory neuropathy or encephalomyelitis (average titer, 4,592 U/ml). In the group with nonparaneoplastic small cell lung cancer (low anti-Hu titer) there was a predominance of women (5 women: 2 men), and all patients had "limited" disease when diagnosed. In the antibody-negative group the sex ratio was 16 women to 21 men and 51% of the patients had "extensive" disease. None of the 7 patients with a low-titer anti-Hu antibody developed a paraneoplastic syndrome by the time of writing. The anti-Hu antibody appears, when present, to be a good marker for small cell lung cancer and, when present at high titer, for small cell lung cancer associated with a paraneoplastic syndrome.
我们检测了50名正常受试者、44名未合并副肿瘤性疾病的小细胞肺癌患者以及25名合并副肿瘤性感觉神经病变、脑脊髓炎或两者皆有的小细胞肺癌患者血清中抗Hu抗体的存在情况。采用抗生物素蛋白-生物素免疫过氧化物酶法和高灵敏度定量蛋白质印迹分析，在50份正常人血清中未检测到抗Hu抗体。44名无副肿瘤综合征的小细胞肺癌患者中，有7名在蛋白质印迹分析中可检测到抗Hu抗体水平（平均滴度为76 U/ml）。这些水平显著低于25名患有小细胞肺癌并伴有副肿瘤性感觉神经病变或脑脊髓炎患者的平均滴度（平均滴度为4592 U/ml）。在非副肿瘤性小细胞肺癌组（抗Hu滴度低）中，女性占多数（5名女性：2名男性），且所有患者确诊时均为“局限性”疾病。在抗体阴性组中，男女比例为16名女性比21名男性，51%的患者患有“广泛性”疾病。截至撰写本文时，7名抗Hu抗体滴度低的患者均未发生副肿瘤综合征。抗Hu抗体一旦出现，似乎是小细胞肺癌的良好标志物，而高滴度时，则是与副肿瘤综合征相关的小细胞肺癌的良好标志物。
