Yen H C, Marrs B
J Bacteriol. 1976 May;126(2):619-29. doi: 10.1128/jb.126.2.619-629.1976.
The recently discovered gene transfer system of Rhodopseudomonas capsulata was used to construct a genetic map of a region concerned with bacteriochlorophyll and carotenoid production. Mutants blocked in the biosynthesis of these compounds were isolated, and each was characterized on the basis of pigments accumulated during growth under low pO2. One-point, two-point, three-point, and ratio test crosses were performed between various mutant strains, and the results were amenable to conventional genetic analyses. A mapping function was found that related cotransfer frequency to map distance. Seven clusters of mutations, five affecting carotenoid and two affecting bacteriochlorophyll biosynthesis, were arranged in one linkage group. Each cluster of mutations is thought to represent a gene. The length of the mapped region is estimated to be less than 1% of the genome. Cotransfer is observed between markers separated by about 5 to 10 genes.
利用最近发现的荚膜红假单胞菌基因转移系统构建了与细菌叶绿素和类胡萝卜素产生相关区域的遗传图谱。分离出了在这些化合物生物合成过程中受阻的突变体,并根据低pO₂条件下生长期间积累的色素对每个突变体进行了表征。在各种突变菌株之间进行了单点、两点、三点和比率测试杂交,结果适合进行常规遗传分析。发现了一种将共转移频率与图谱距离相关联的定位函数。七个突变簇,五个影响类胡萝卜素,两个影响细菌叶绿素生物合成,排列在一个连锁群中。每个突变簇被认为代表一个基因。所绘制区域的长度估计小于基因组的1%。在相隔约5到10个基因的标记之间观察到共转移。