Dieminger L, Vogt W, Lynen R
Z Immunitatsforsch Immunobiol. 1976 Nov;152(3):231-43.
Factor D has been purified by gel and ion exchange chromatographies, and by ultrafiltration through different membranes. The final preparation appeared pure in various analytical tests. The molecular weight of human D is 21,500 according to gel chromatography, the isoelectric point was found at pH 7.8. Factor D is an active esterolytic enzyme, it cleaves N-alpha-acetyl-L-lysine methyl ester and N-alpha-acetyl-L-glycyl-L-lysine methyl ester. Both peptide esters inhibit the hydrolytic activation of factor B by D in the presence of cobra venom factor. D is also inhibited by diisopropyl-fluorophosphate and by penylmethyl-sulfonyl-flouride.
D因子已通过凝胶色谱、离子交换色谱以及通过不同膜的超滤进行了纯化。最终制剂在各种分析测试中均显示为纯品。根据凝胶色谱法，人D因子的分子量为21,500，其等电点为pH 7.8。D因子是一种活性酯解酶，它能切割N-α-乙酰-L-赖氨酸甲酯和N-α-乙酰-L-甘氨酰-L-赖氨酸甲酯。在眼镜蛇毒因子存在的情况下，这两种肽酯均能抑制D因子对B因子的水解激活作用。D因子也受到二异丙基氟磷酸酯和苯甲基磺酰氟的抑制。
