Teyssier C, Jumas-Bilak E, Marchandin H, Jean-Pierre H, Jeannot J L, Dusart G, Foulongne V, Siméon de Buochberg M
Laboratoire de bactériologie, faculté de pharmacie, 15, avenue Charles-Flahault, 34093 cedex 5, Montpellier, France.
Pathol Biol (Paris). 2003 Feb;51(1):5-12. doi: 10.1016/s0369-8114(02)00361-9.
Two species of medical interest belong to the genus Ochrobactrum, Ochrobactrum anthropi and Ochrobactrum intermedium. They are members of the microbiota of soil and an increasing number of works report the isolation of O. anthropi from clinical specimen, especially from immunocompromised patients and nosocomial infection. Involving of each species in human infection is poorly estimated due to unclear differential phenotypic characters. We performed 16S rDNA sequencing for identification of 20 clinical isolates of Ochrobactrum sp. to the species level. Then, we studied the phenotype of each isolate especially, morphology, culture onto different media and at different temperatures, biochemical characters and antibiotics resistance pattern. Colony morphology after growth onto Trypticase-Soy and McConkey agar, culture at 45 degrees C onto Trypticase-Soja agar, presence of urease, and netilmycin, tobramycin and colistin resistance allowed identification of species. Ribotyping using HindIII and EcoRI gave a supplementary criterion for species determination but did not allow typing at the infra-species level. In contrast, Pulsed-Field Gel Electrophoresis showed high degree of polymorphism between strains and proved the clonality of certain isolates. Thus, this method could be a useful tool for molecular epidemiology of Ochrobactrum infections.
有两种医学上感兴趣的菌属于苍白杆菌属，即嗜人苍白杆菌和中间苍白杆菌。它们是土壤微生物群的成员，越来越多的研究报告从临床标本中分离出嗜人苍白杆菌，特别是从免疫功能低下的患者和医院感染中分离得到。由于不同的表型特征不明确，每种菌在人类感染中的作用难以评估。我们进行了16S rDNA测序，以将20株苍白杆菌属临床分离株鉴定到种水平。然后，我们特别研究了每株分离株的表型，包括形态、在不同培养基和不同温度下的培养情况、生化特征以及抗生素耐药模式。在胰蛋白胨大豆琼脂和麦康凯琼脂上生长后的菌落形态、在45℃下于胰蛋白胨大豆琼脂上培养、脲酶的存在以及对奈替米星、妥布霉素和黏菌素的耐药性有助于菌种鉴定。使用HindIII和EcoRI进行核糖分型为菌种鉴定提供了补充标准，但不能在种以下水平进行分型。相比之下，脉冲场凝胶电泳显示菌株之间具有高度多态性，并证明了某些分离株的克隆性。因此，该方法可能是苍白杆菌感染分子流行病学的有用工具。
